Hematopoietic progenitor kinase 1 down-regulates the oncogenic receptor tyrosine kinase AXL in pancreatic cancer

The oncogenic receptor tyrosine kinase Axl is overexpressed in Cancer and plays an important role in carcinomas of multiple organs. However, the mechanisms of Axl overexpression in Cancer remain unclear. In this study, using HEK293T, Panc-1, and Panc-28 cells and samples of human pancreatic intraepithelial neoplasia (PanIN), along with several biochemical approaches and immunofluorescence microscopy analyses, we sought to investigate the mechanisms that regulate Axl over-expression in pancreatic ductal adenocarcinoma (PDAC). We found that Axl interacts with hematopoietic progenitor kinase 1 (HPK1) and demonstrate that HPK1 down-regulates Axl and decreases its half-life. The HPK1-mediated Axl degradation was inhibited by the endocytic pathway inhibitors leupeptin, bafilomycin A1, and monensin. HPK1 accelerated the movement of Axl from the plasma membrane to endosomes in pancreatic Cancer cells treated with the Axl ligand growth arrest-specific 6 (GAS6). Moreover, HPK1 increased the binding of Axl to the Cbl proto-oncogene (c-Cbl); promoted Axl ubiquitination; decreased AXL-mediated signaling, including phospho-AKT and phospho-ERK signaling; and decreased the invasion capability of PDAC cells. Importantly, we show that Axl expression inversely correlates with HPK1 expression in human PanINs and that patients whose tumors have low HPK1 and high Axl expression levels have shorter survival than those with low Axl or high HPK1 expression (p < 0.001). Our results suggest that HPK1 is a tumor suppressor that targets Axl for degradation via the endocytic pathway. HPK1 loss of function may contribute to Axl overexpression and thereby enhance AXL-dependent downstream signaling and tumor invasion in PDAC.

Axl; Gas6; HPK1; c-Cbl; endocytosis; kinase signaling; pancreatic cancer; protein degradation; receptor tyrosine kinase; tumor suppressor gene; ubiquitylation (ubiquitination).