2. Academic Validation
  3. A Sequential Dual-Lock Strategy for Photoactivatable Chemiluminescent Probes Enabling Bright Duplex Optical Imaging

A Sequential Dual-Lock Strategy for Photoactivatable Chemiluminescent Probes Enabling Bright Duplex Optical Imaging

  • Angew Chem Int Ed Engl. 2020 Jun 2;59(23):9059-9066. doi: 10.1002/anie.202000165.
Yutao Zhang 1,  Chenxu Yan 1,  Chao Wang 2,  Zhiqian Guo 1,  Xiaogang Liu 2,  Wei-Hong Zhu 1
Affiliations

Affiliations

  • 1 Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science & Technology, Shanghai, 200237, China.
  • 2 Fluorescence Research Group, Science and Math Cluster, Singapore University of Technology and Design, Singapore, 487372, Singapore.
PMID: 31984609 DOI: 10.1002/anie.202000165
Abstract

Chemiluminescence (CL)-based technologies have revolutionized in vivo monitoring of biomolecules. However, significant technical hurdles have limited the achievement of trigger-controlled, bright, and enriched CL signal. Herein, a dual-lock strategy uses sequence-dependent triggers for bright optical imaging with real-time fluorescent signal and ultra-sensitive CL signal. These probes can obtain an analyte-triggered accumulation of stable pre-chemiluminophore with aggregation-induced emission (AIE), and then the pre-chemiluminophore exhibits a rapid photooxidation process (1,2-dioxetane generation) by TICT-based free-radical addition, thereby achieving an enrichment and bright CL signal. The dual-lock strategy expands the in vivo toolbox for highly accurate analysis and has for the first time allowed access to accurately sense and trace biomolecules with high-resolution, dual-mode of chemo-fluoro-luminescence, and three-dimensional (3D) imaging in living Animals.

Keywords

aggregation-induced emission; chemiluminescent probes; dual-mode bioimaging; fluorescence probes; sequential dual-lock.

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