1. Academic Validation
  2. Methylcobalamin-Loaded PLCL Conduits Facilitate the Peripheral Nerve Regeneration

Methylcobalamin-Loaded PLCL Conduits Facilitate the Peripheral Nerve Regeneration

  • Macromol Biosci. 2020 Mar;20(3):e1900382. doi: 10.1002/mabi.201900382.
Deteng Zhang 1 2 Weichao Yang 3 Chunyang Wang 3 Honghao Zheng 1 Zhizhou Liu 1 Zhehan Chen 1 Changyou Gao 1 2
Affiliations

Affiliations

  • 1 MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China.
  • 2 Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou, 310058, China.
  • 3 Department of Orthopedic Surgery, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, 200233, China.
Abstract

The feasible fabrication of nerve guidance conduits (NGCs) with good biological performance is important for translation in clinics. In this study, poly(d,l-lactide-co-caprolactone) (PLCL) films loaded with various amounts (wt; 5%, 15%, 25%) of methylcobalamin (MeCbl) are prepared, and are further rolled and sutured to obtain MeCbl-loaded NGCs. The MeCbl can be released in a sustainable manner up to 21 days. The proliferation and elongation of Schwann cells, and the proliferation of Neuro2a cells are enhanced on these MeCbl-loaded films. The MeCbl-loaded NGCs are implanted into rats to induce the regeneration of 10 mm amputated sciatic nerve defects, showing the ability to facilitate the recovery of motor and sensory function, and to promote myelination in peripheral nerve regeneration. In particular, the 15% MeCbl-loaded PLCL conduit exhibits the most satisfactory recovery of sciatic nerves in rats with the largest diameter and thickest myelinated fibers.

Keywords

Schwann cells; methylcobalamin; nerve guidance conduit; peripheral nerve regeneration; poly( d, l-lactide-co-caprolactone).

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