Determination of 2,4-decadienal in edible oils using reversed-phase liquid chromatography and its application as an alternative indicator of lipid oxidation

As a major product of linoleic acid-rich oils, 2,4-decadienal has unique reactivity that may be potentially toxic to human body. In this study, a reliable reversed-phase liquid chromatography method for the determination of carbonyls was developed, and 2,4-decadienal as the target aldehyde was validated. Furthermore, the possibility of 2,4-decadienal as a lipid oxidation marker was evaluated. The optimal sample pretreatment method was extraction by 2 mL of acetonitrile three times, followed by derivatization at 40 °C for 30 min. The method was linear, sensitive, and accurate with detection and quantification limits of 15 and 50 nmol/L, respectively, and had good average recoveries for 2,4-decadienal in oil samples. In tested edible oils, during heating at 180 °C, the level of 2,4-decadienal rose faster than other aldehydes, including one of the characteristic aldehydes, hexanal. Moreover, good linear relationships between the 2,4-decadienal content and other oxidation indices (R² = 0.858 to 0.984 for the anisidine value; R² = 0.876 to 0.986 for the total oxidation value) were observed in sunflower and corn oils under 8 hr heating at three temperatures (120, 150, and 180 °C), indicating that 2,4-decadienal can predict the oxidation of oil. PRACTICAL APPLICATION: 2,4-Decadienal is a toxic aldehyde produced by the oxidation of linoleic acid-rich oils, which is closely related to human health. This work is the first to demonstrate that 2,4-decadienal can be used as an alternative oxidation indicator for linoleic acid-rich oils and is of great significance for the quality control of edible oil in the food industry.

2,4-decadienal; edible oils; method validation; oxidative indicator; quantitative determination.