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  3. Kinesin-7 CENP-E regulates chromosome alignment and genome stability of spermatogenic cells

Kinesin-7 CENP-E regulates chromosome alignment and genome stability of spermatogenic cells

  • Cell Death Discov. 2020 Apr 20;6:25. doi: 10.1038/s41420-020-0261-8.
Zhen-Yu She  # 1 2,  Kai-Wei Yu 1 2,  Ning Zhong 1 2,  Yu Xiao 1 2,  Ya-Lan Wei 3 4,  Yang Lin 1 2,  Yue-Ling Li 1 2,  Ming-Hui Lu 1 2
Affiliations

Affiliations

  • 1 Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, 350122 China.
  • 2 Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University, Fuzhou, Fujian, 350122 China.
  • 3 Fujian Obstetrics and Gynecology Hospital, Fuzhou, Fujian, 350001 China.
  • 4 Medical Research Center, Fujian Provincial Children's Hospital, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, 350001 China.
  • # Contributed equally.
PMID: 32351712 DOI: 10.1038/s41420-020-0261-8
Abstract

Kinesin-7 CENP-E is an essential kinetochore motor required for chromosome alignment and congression. However, the specific functions of CENP-E in the spermatogenic cells during spermatogenesis remain unknown. In this study, we find that CENP-E proteins are expressed in the spermatogonia, spermatocytes, and the elongating spermatids. CENP-E inhibition by specific inhibitor GSK923295 results in the disruption of spermatogenesis and cell cycle arrest of spermatogenic cells. Both spermatogonia and spermatocytes are arrested in metaphase and several chromosomes are not aligned at the equatorial plate. We find that CENP-E inhibition leads to chromosome misalignment, the spindle disorganization, and the formation of the aneuploidy cells. Furthermore, the inhibition of CENP-E results in the defects in the formation of spermatids, including the sperm head condensation and the sperm tail formation. We have revealed that kinesin-7 CENP-E is essential for chromosome alignment and genome stability of the spermatogenic cells.

Keywords

Infertility; Kinesin.

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