GSK-923295

Name: GSK-923295
Brand: MedChemExpress
SKU: HY-10299
Rating: 5.0 (40 reviews)

Cat. No.: HY-10299 Purity: 99.95%: Data Sheet
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GSK-923295 is a special, allosteric inhibitor of centromere-associated protein-E (CENP-E) kinesin motor ATPase activity, with K_i of 3.2±0.2 nM and 1.6± 0.1 nM for human and canine, respectively.

For research use only. We do not sell to patients.

CAS No. : 1088965-37-0

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10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
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10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
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Based on 40 publication(s) in Google Scholar

40 Publications Citing Use of MCE GSK-923295

: GSK-923295 purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2015 May 28;361(1):97-103. [Abstract]; Apoptotic effects of GSK923295 on MESSA and MESSA Dx5 cells. MESSA and MESSA Dx5 cells are treated with Verapamil (20 μM), GSK923295 (4 μM), and combination GSK923295 (4 μM) plus Verapamil (20 μM) for 24 h. Actin is used as loading control.

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Kinesin-5/Eg5/KSP Kinesin-6 Kinesin-7/CENP-E Kinesin-12 Kinesin-14 Kinesin-13

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Description

IC₅₀ & Target^[1]

CENP-E

1.6 nM (Ki, Canine CENP-E)

Cellular Effect

Cell Line	Type	Value	Description	References
5637	GI₅₀	23 nM Compound: GSK923295	Anticancer activity against human 5637 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human 5637 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
639-V	GI₅₀	27 nM Compound: GSK923295	Anticancer activity against human 639-V cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human 639-V cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
647-V	GI₅₀	72 nM Compound: GSK923295	Anticancer activity against human 647-V cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human 647-V cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
786-0	GI₅₀	45 nM Compound: GSK923295	Anticancer activity against human 786-0 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human 786-0 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
A 172	GI₅₀	27 nM Compound: GSK923295	Anticancer activity against human A-172 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human A-172 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
A-375	GI₅₀	26 nM Compound: GSK923295	Anticancer activity against human A-375 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human A-375 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
A-427	GI₅₀	97 nM Compound: GSK923295	Anticancer activity against human A-427 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human A-427 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
A-431	GI₅₀	21 nM Compound: GSK923295	Anticancer activity against human A-431 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human A-431 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
A101D	GI₅₀	17 nM Compound: GSK923295	Anticancer activity against human A101D cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human A101D cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
A204	GI₅₀	18 nM Compound: GSK923295	Anticancer activity against human A204 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human A204 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
A2780	GI₅₀	13 nM Compound: GSK923295	Anticancer activity against human A2780 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human A2780 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
A498	GI₅₀	81 nM Compound: GSK923295	Anticancer activity against human A498 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human A498 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
A549	GI₅₀	21 nM Compound: GSK923295	Anticancer activity against human A549 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human A549 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
A673	GI₅₀	23 nM Compound: GSK923295	Anticancer activity against human A673 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human A673 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
ACHN	GI₅₀	61 nM Compound: GSK923295	Anticancer activity against human ACHN cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human ACHN cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
AGS	GI₅₀	29 nM Compound: GSK923295	Anticancer activity against human AGS cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human AGS cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
AN3-CA	GI₅₀	23 nM Compound: GSK923295	Anticancer activity against human AN3-CA cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human AN3-CA cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
ARH-77	GI₅₀	34 nM Compound: GSK923295	Anticancer activity against human ARH-77 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human ARH-77 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
BC-3	GI₅₀	88 nM Compound: GSK923295	Anticancer activity against human BC-3 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human BC-3 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
BE(2)-C	GI₅₀	93 nM Compound: GSK923295	Anticancer activity against human BE2C cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human BE2C cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
BFTC-905	GI₅₀	30 nM Compound: GSK923295	Anticancer activity against human BFTC-905 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human BFTC-905 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
BHT-101	GI₅₀	100 nM Compound: GSK923295	Anticancer activity against human BHT-101 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human BHT-101 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
BPH-1	GI₅₀	52 nM Compound: GSK923295	Anticancer activity against human BPH-1 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human BPH-1 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
BT-20	GI₅₀	32 nM Compound: GSK923295	Anticancer activity against human BT-20 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human BT-20 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
BT-474	GI₅₀	110 nM Compound: GSK923295	Anticancer activity against human BT-474 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human BT-474 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
BV-173	GI₅₀	56 nM Compound: GSK923295	Anticancer activity against human BV-173 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human BV-173 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
BeWo	GI₅₀	26 nM Compound: GSK923295	Anticancer activity against human BeWo cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human BeWo cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
C-33-A	GI₅₀	47 nM Compound: GSK923295	Anticancer activity against human C-33-A cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human C-33-A cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
C-4-II	GI₅₀	46 nM Compound: GSK923295	Anticancer activity against human C-4-II cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human C-4-II cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
C3A	GI₅₀	54 nM Compound: GSK923295	Anticancer activity against human C3A cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human C3A cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
CAKI-2	GI₅₀	25 nM Compound: GSK923295	Anticancer activity against human CAKI-2 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human CAKI-2 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
CAL-27	GI₅₀	47 nM Compound: GSK923295	Anticancer activity against human CAL-27 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human CAL-27 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
CAL-54	GI₅₀	49 nM Compound: GSK923295	Anticancer activity against human CAL-54 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human CAL-54 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
CAL-62	GI₅₀	30 nM Compound: GSK923295	Anticancer activity against human CAL-62 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human CAL-62 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
CCF-STTG1	GI₅₀	24 nM Compound: GSK923295	Anticancer activity against human CCF-STTG1 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human CCF-STTG1 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
CESS	GI₅₀	19 nM Compound: GSK923295	Anticancer activity against human CESS cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human CESS cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
CGTH-W-1	GI₅₀	30 nM Compound: GSK923295	Anticancer activity against human CGTH-W-1 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human CGTH-W-1 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
CHL-1	GI₅₀	24 nM Compound: GSK923295	Anticancer activity against human CHL-1 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human CHL-1 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
CHP-212	GI₅₀	34 nM Compound: GSK923295	Anticancer activity against human CHP-212 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human CHP-212 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
COLO 201	GI₅₀	24 nM Compound: GSK923295	Anticancer activity against human COLO201 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human COLO201 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
COLO 205	GI₅₀	25 nM Compound: GSK923295	Anticancer activity against human COLO 205 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human COLO 205 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
COLO 320DM	GI₅₀	306 nM Compound: GSK923295	Anticancer activity against human COLO 320DM cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human COLO 320DM cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
COLO-320-HSR	GI₅₀	153 nM Compound: GSK923295	Anticancer activity against human COLO 320HSR cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human COLO 320HSR cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
CRO-AP2	GI₅₀	51 nM Compound: GSK923295	Anticancer activity against human CRO-AP2 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human CRO-AP2 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
CWR22R	GI₅₀	29 nM Compound: GSK923295	Anticancer activity against human 22Rv1 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human 22Rv1 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
Capan-2	GI₅₀	119 nM Compound: GSK923295	Anticancer activity against human Capan-2 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human Capan-2 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
D283 Med	GI₅₀	28 nM Compound: GSK923295	Anticancer activity against human D283 Med cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human D283 Med cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
DB	GI₅₀	21 nM Compound: GSK923295	Anticancer activity against human DB cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human DB cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
DBTRG-05MG	GI₅₀	17 nM Compound: GSK923295	Anticancer activity against human DBTRG-05MG cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human DBTRG-05MG cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
DLD-1	GI₅₀	60 nM Compound: GSK923295	Anticancer activity against human DLD-1 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human DLD-1 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
DU-145	GI₅₀	23 nM Compound: GSK923295	Anticancer activity against human DU-145 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human DU-145 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
DU-4475	GI₅₀	147 nM Compound: GSK923295	Anticancer activity against human DU-4475 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human DU-4475 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
Detroit 562	GI₅₀	35 nM Compound: GSK923295	Anticancer activity against human Detroit 562 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human Detroit 562 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
DoTc2-4510	GI₅₀	33 nM Compound: GSK923295	Anticancer activity against human DoTc2-4510 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human DoTc2-4510 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
EFM-19	GI₅₀	28 nM Compound: GSK923295	Anticancer activity against human EFM-19 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human EFM-19 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
EKVX	GI₅₀	28 nM Compound: GSK923295	Anticancer activity against human EKVX cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human EKVX cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
ES-2	GI₅₀	30 nM Compound: GSK923295	Anticancer activity against human ES2 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human ES2 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
FaDu	GI₅₀	32 nM Compound: GSK923295	Anticancer activity against human FaDu cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human FaDu cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
G-401	GI₅₀	26 nM Compound: GSK923295	Anticancer activity against human G-401 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human G-401 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
G-402	GI₅₀	31 nM Compound: GSK923295	Anticancer activity against human G-402 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human G-402 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
GCT	GI₅₀	23 nM Compound: GSK923295	Anticancer activity against human GCT cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human GCT cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
H4	GI₅₀	31 nM Compound: GSK923295	Anticancer activity against human H4 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human H4 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
HCC 2998	GI₅₀	29 nM Compound: GSK923295	Anticancer activity against human HCC 2998 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human HCC 2998 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
HCC1143	GI₅₀	27 nM Compound: GSK923295	Anticancer activity against human HCC1143 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human HCC1143 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
HCC1954	GI₅₀	18 nM Compound: GSK923295	Anticancer activity against human HCC1954 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human HCC1954 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
HCC70	GI₅₀	24 nM Compound: GSK923295	Anticancer activity against human HCC70 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human HCC70 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
HCT-116	GI₅₀	31 nM Compound: GSK923295	Anticancer activity against human HCT-116 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human HCT-116 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
HCT-15	GI₅₀	151 nM Compound: GSK923295	Anticancer activity against human HCT-15 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human HCT-15 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
HCT-8	GI₅₀	35 nM Compound: GSK923295	Anticancer activity against human HCT-8 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human HCT-8 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
HEC-1-A	GI₅₀	50 nM Compound: GSK923295	Anticancer activity against human HEC-1-A cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human HEC-1-A cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
HN5	GI₅₀	118 nM Compound: GSK923295	Anticancer activity against human HN5 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human HN5 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
HOP-62	GI₅₀	20 nM Compound: GSK923295	Anticancer activity against human HOP-62 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human HOP-62 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
HOS	GI₅₀	27 nM Compound: GSK923295	Anticancer activity against human HOS cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human HOS cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
HPAF-II	GI₅₀	5026 nM Compound: GSK923295	Anticancer activity against human HPAF-II cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human HPAF-II cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
HT-1080	GI₅₀	24 nM Compound: GSK923295	Anticancer activity against human HT-1080 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human HT-1080 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
HT-1376	GI₅₀	33 nM Compound: GSK923295	Anticancer activity against human HT-1376 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human HT-1376 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
HT-29	GI₅₀	14 nM Compound: GSK923295	Anticancer activity against human HT-29 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human HT-29 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
HT-3	GI₅₀	10000 nM Compound: GSK923295	Anticancer activity against human HT-3 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human HT-3 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
HeLa	GI₅₀	20 nM Compound: GSK923295	Anticancer activity against human HeLa cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human HeLa cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
Hep 3B2	GI₅₀	164 nM Compound: GSK923295	Anticancer activity against human Hep3B cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human Hep3B cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
HepG2	GI₅₀	31 nM Compound: GSK923295	Anticancer activity against human HepG2 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human HepG2 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
HuP-T4	GI₅₀	158 nM Compound: GSK923295	Anticancer activity against human HuP-T4 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human HuP-T4 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
HuT78	GI₅₀	40 nM Compound: GSK923295	Anticancer activity against human HuT78 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human HuT78 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
IGROV-1	GI₅₀	31 nM Compound: GSK923295	Anticancer activity against human IGROV-1 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human IGROV-1 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
J82	GI₅₀	114 nM Compound: GSK923295	Anticancer activity against human J82 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human J82 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
JEG-3	GI₅₀	25 nM Compound: GSK923295	Anticancer activity against human JEG-3 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human JEG-3 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
K562	GI₅₀	25 nM Compound: GSK923295	Anticancer activity against human K562 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human K562 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
KM12	GI₅₀	56 nM Compound: GSK923295	Anticancer activity against human KM12 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human KM12 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
LNCaP	GI₅₀	76 nM Compound: GSK923295	Anticancer activity against human LNCaP cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human LNCaP cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
LOX IMVI	GI₅₀	25 nM Compound: GSK923295	Anticancer activity against human LOX IMVI cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human LOX IMVI cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
LS-1034	GI₅₀	97 nM Compound: GSK923295	Anticancer activity against human LS1034 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human LS1034 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
M14	GI₅₀	27 nM Compound: GSK923295	Anticancer activity against human M14 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human M14 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
MC-CAR	GI₅₀	86 nM Compound: GSK923295	Anticancer activity against human MC-CAR cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human MC-CAR cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
MC-IXC	GI₅₀	27 nM Compound: GSK923295	Anticancer activity against human MC-IXC cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human MC-IXC cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
MCF-10A	GI₅₀	16 nM Compound: GSK923295	Anticancer activity against human MCF-10A cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human MCF-10A cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
MCF7	GI₅₀	29 nM Compound: GSK923295	Anticancer activity against human MCF7 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human MCF7 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
MDA-MB-175-VII	GI₅₀	23 nM Compound: GSK923295	Anticancer activity against human MDA-MB-175-VII cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human MDA-MB-175-VII cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
MDA-MB-231	GI₅₀	5015 nM Compound: GSK923295	Anticancer activity against human MDA-MB-231 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human MDA-MB-231 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
MDA-MB-435	GI₅₀	30 nM Compound: GSK923295	Anticancer activity against human MDA-MB-435 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human MDA-MB-435 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
MDA-MB-453	GI₅₀	27 nM Compound: GSK923295	Anticancer activity against human MDA-MB-453 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human MDA-MB-453 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
MDA-MB-468	GI₅₀	25 nM Compound: GSK923295	Anticancer activity against human MDA-MB-468 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human MDA-MB-468 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
MES-SA	GI₅₀	65 nM Compound: GSK923295	Anticancer activity against human MES-SA cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human MES-SA cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
MIA PaCa-2	GI₅₀	23 nM Compound: GSK923295	Anticancer activity against human MIA PaCa-2 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human MIA PaCa-2 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
MOLT-16	GI₅₀	47 nM Compound: GSK923295	Anticancer activity against human MOLT-16 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human MOLT-16 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
MV4-11	GI₅₀	29 nM Compound: GSK923295	Anticancer activity against human MV4-11 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human MV4-11 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
MX1	GI₅₀	30 nM Compound: GSK923295	Anticancer activity against human MX1 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human MX1 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
Malme-3M	GI₅₀	51 nM Compound: GSK923295	Anticancer activity against human Malme-3M cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human Malme-3M cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
NCI-H1299	GI₅₀	52 nM Compound: GSK923295	Anticancer activity against human NCI-H1299 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human NCI-H1299 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
NCI-H146	GI₅₀	5226 nM Compound: GSK923295	Anticancer activity against human NCI-H146 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human NCI-H146 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
NCI-H157	GI₅₀	37 nM Compound: GSK923295	Anticancer activity against human NCI-H157 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human NCI-H157 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
NCI-H187	GI₅₀	68 nM Compound: GSK923295	Anticancer activity against human NCI-H187 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human NCI-H187 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
NCI-H2052	GI₅₀	44 nM Compound: GSK923295	Anticancer activity against human NCI-H2052 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human NCI-H2052 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
NCI-H209	GI₅₀	20 nM Compound: GSK923295	Anticancer activity against human NCI-H209 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human NCI-H209 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
NCI-H2122	GI₅₀	29 nM Compound: GSK923295	Anticancer activity against human NCI-H2122 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human NCI-H2122 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
NCI-H2170	GI₅₀	25 nM Compound: GSK923295	Anticancer activity against human NCI-H2170 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human NCI-H2170 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
NCI-H2171	GI₅₀	24 nM Compound: GSK923295	Anticancer activity against human NCI-H2171 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human NCI-H2171 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
NCI-H23	GI₅₀	27 nM Compound: GSK923295	Anticancer activity against human NCI-H23 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human NCI-H23 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
NCI-H292	GI₅₀	31 nM Compound: GSK923295	Anticancer activity against human NCI-H292 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human NCI-H292 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
NCI-H295R	GI₅₀	298 nM Compound: GSK923295	Anticancer activity against human NCI-H295R cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human NCI-H295R cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
NCI-H358	GI₅₀	18 nM Compound: GSK923295	Anticancer activity against human NCI-H358 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human NCI-H358 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
NCI-H460	GI₅₀	29 nM Compound: GSK923295	Anticancer activity against human NCI-H460 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human NCI-H460 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
NCI-H508	GI₅₀	32 nM Compound: GSK923295	Anticancer activity against human NCI-H508 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human NCI-H508 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
NCI-H520	GI₅₀	34 nM Compound: GSK923295	Anticancer activity against human NCI-H520 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human NCI-H520 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
NCI-H526	GI₅₀	88 nM Compound: GSK923295	Anticancer activity against human NCI-H526 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human NCI-H526 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
NCI-H596	GI₅₀	5017 nM Compound: GSK923295	Anticancer activity against human NCI-H596 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human NCI-H596 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
NCI-H630	GI₅₀	47 nM Compound: GSK923295	Anticancer activity against human NCI-H630 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human NCI-H630 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
NCI-H661	GI₅₀	22 nM Compound: GSK923295	Anticancer activity against human NCI-H661 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human NCI-H661 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
NCI-H716	GI₅₀	174 nM Compound: GSK923295	Anticancer activity against human NCI-H716 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human NCI-H716 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
NCI-H747	GI₅₀	163 nM Compound: GSK923295	Anticancer activity against human NCI-H747 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human NCI-H747 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
NCI-H82	GI₅₀	18 nM Compound: GSK923295	Anticancer activity against human NCI-H82 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human NCI-H82 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
NCI-N87	GI₅₀	29 nM Compound: GSK923295	Anticancer activity against human NCI-N87 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human NCI-N87 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
NCI/ADR-RES	GI₅₀	3962 nM Compound: GSK923295	Anticancer activity against human NCI/ADR-RES cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human NCI/ADR-RES cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
OV-90	GI₅₀	61 nM Compound: GSK923295	Anticancer activity against human OV-90 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human OV-90 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
OVCAR-3	GI₅₀	42 nM Compound: GSK923295	Anticancer activity against human OVCAR-3 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human OVCAR-3 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
OVCAR-4	GI₅₀	30 nM Compound: GSK923295	Anticancer activity against human OVCAR-4 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human OVCAR-4 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
OVCAR-5	GI₅₀	34 nM Compound: GSK923295	Anticancer activity against human OVCAR-5 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human OVCAR-5 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
OVCAR-8	GI₅₀	50 nM Compound: GSK923295	Anticancer activity against human OVCAR-8 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human OVCAR-8 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
PC-3	GI₅₀	28 nM Compound: GSK923295	Anticancer activity against human PC-3 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human PC-3 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
RD	GI₅₀	28 nM Compound: GSK923295	Anticancer activity against human RD cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human RD cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
RKO	GI₅₀	53 nM Compound: GSK923295	Anticancer activity against human RKO cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human RKO cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
RL	GI₅₀	37 nM Compound: GSK923295	Anticancer activity against human RL cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human RL cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
RL95-2	GI₅₀	49 nM Compound: GSK923295	Anticancer activity against human RL95-2 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human RL95-2 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
RPMI-2650	GI₅₀	36 nM Compound: GSK923295	Anticancer activity against human RPMI-2650 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human RPMI-2650 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
RPMI-6666	GI₅₀	88 nM Compound: GSK923295	Anticancer activity against human RPMI-6666 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human RPMI-6666 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
RPMI-8226	GI₅₀	27 nM Compound: GSK923295	Anticancer activity against human RPMI-8226 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human RPMI-8226 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
RXF 393	GI₅₀	32 nM Compound: GSK923295	Anticancer activity against human RXF 393 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human RXF 393 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
Raji	GI₅₀	41 nM Compound: GSK923295	Anticancer activity against human Raji cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human Raji cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SAOS-2	GI₅₀	41 nM Compound: GSK923295	Anticancer activity against human SAOS-2 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SAOS-2 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SCC-15	GI₅₀	63 nM Compound: GSK923295	Anticancer activity against human SCC-15 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SCC-15 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SCC-25	GI₅₀	23 nM Compound: GSK923295	Anticancer activity against human SCC-25 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SCC-25 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SCC-4	GI₅₀	28 nM Compound: GSK923295	Anticancer activity against human SCC-4 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SCC-4 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SCC-9	GI₅₀	24 nM Compound: GSK923295	Anticancer activity against human SCC-9 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SCC-9 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SCaBER	GI₅₀	54 nM Compound: GSK923295	Anticancer activity against human SCaBER cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SCaBER cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SF-268	GI₅₀	26 nM Compound: GSK923295	Anticancer activity against human SF-268 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SF-268 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SF-295	GI₅₀	35 nM Compound: GSK923295	Anticancer activity against human SF-295 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SF-295 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SF-539	GI₅₀	28 nM Compound: GSK923295	Anticancer activity against human SF-539 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SF-539 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SH-4	GI₅₀	44 nM Compound: GSK923295	Anticancer activity against human SH-4 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SH-4 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SHP77	GI₅₀	86 nM Compound: GSK923295	Anticancer activity against human SHP77 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SHP77 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SJRH30	GI₅₀	26 nM Compound: GSK923295	Anticancer activity against human SJRH30 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SJRH30 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SK-LMS-1	GI₅₀	30 nM Compound: GSK923295	Anticancer activity against human SK-LMS-1 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SK-LMS-1 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SK-MEL-28	GI₅₀	31 nM Compound: GSK923295	Anticancer activity against human SK-MEL-28 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SK-MEL-28 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SK-MEL3	GI₅₀	30 nM Compound: GSK923295	Anticancer activity against human SK-MEL3 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SK-MEL3 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SK-N-AS	GI₅₀	38 nM Compound: GSK923295	Anticancer activity against human SK-N-AS cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SK-N-AS cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SK-N-DZ	GI₅₀	69 nM Compound: GSK923295	Anticancer activity against human SK-N-DZ cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SK-N-DZ cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SK-N-FI	GI₅₀	120 nM Compound: GSK923295	Anticancer activity against human SK-N-FI cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SK-N-FI cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SK-NEP-1	GI₅₀	55 nM Compound: GSK923295	Anticancer activity against human SK-NEP-1 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SK-NEP-1 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SK-OV-3	GI₅₀	26 nM Compound: GSK923295	Anticancer activity against human SK-OV-3 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SK-OV-3 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SK-UT-1	GI₅₀	23 nM Compound: GSK923295	Anticancer activity against human SK-UT-1 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SK-UT-1 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SN12C	GI₅₀	29 nM Compound: GSK923295	Anticancer activity against human SN12C cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SN12C cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SNB-19	GI₅₀	28 nM Compound: GSK923295	Anticancer activity against human SNB-19 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SNB-19 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SNB-75	GI₅₀	26 nM Compound: GSK923295	Anticancer activity against human SNB-75 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SNB-75 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SNU-16	GI₅₀	18 nM Compound: GSK923295	Anticancer activity against human SNU-16 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SNU-16 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SNU-387	GI₅₀	32 nM Compound: GSK923295	Anticancer activity against human SNU-387 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SNU-387 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SNU-398	GI₅₀	27 nM Compound: GSK923295	Anticancer activity against human SNU-398 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SNU-398 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SNU-423	GI₅₀	34 nM Compound: GSK923295	Anticancer activity against human SNU-423 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SNU-423 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SNU-449	GI₅₀	236 nM Compound: GSK923295	Anticancer activity against human SNU-449 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SNU-449 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SNU-475	GI₅₀	26 nM Compound: GSK923295	Anticancer activity against human SNU-475 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SNU-475 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SNU-5	GI₅₀	88 nM Compound: GSK923295	Anticancer activity against human SNU-5 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SNU-5 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SNU1	GI₅₀	10000 nM Compound: GSK923295	Anticancer activity against human SNU1 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SNU1 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SR	GI₅₀	29 nM Compound: GSK923295	Anticancer activity against human SR cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SR cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
ST486	GI₅₀	26 nM Compound: GSK923295	Anticancer activity against human ST486 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human ST486 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SW 954	GI₅₀	22 nM Compound: GSK923295	Anticancer activity against human SW 954 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SW 954 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SW 962	GI₅₀	21 nM Compound: GSK923295	Anticancer activity against human SW 962 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SW 962 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SW-620	GI₅₀	47 nM Compound: GSK923295	Anticancer activity against human SW620 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SW620 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SW1088	GI₅₀	32 nM Compound: GSK923295	Anticancer activity against human SW1088 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SW1088 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SW1417	GI₅₀	29 nM Compound: GSK923295	Anticancer activity against human SW1417 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SW1417 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SW1463	GI₅₀	31 nM Compound: GSK923295	Anticancer activity against human SW1463 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SW1463 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SW1783	GI₅₀	34 nM Compound: GSK923295	Anticancer activity against human SW1783 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SW1783 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SW1990	GI₅₀	127 nM Compound: GSK923295	Anticancer activity against human SW1990 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SW1990 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SW48	GI₅₀	25 nM Compound: GSK923295	Anticancer activity against human SW48 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SW48 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SW480	GI₅₀	33 nM Compound: GSK923295	Anticancer activity against human SW480 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SW480 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SW684	GI₅₀	5016 nM Compound: GSK923295	Anticancer activity against human SW684 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SW684 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SW756	GI₅₀	35 nM Compound: GSK923295	Anticancer activity against human SW756 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SW756 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SW780	GI₅₀	30 nM Compound: GSK923295	Anticancer activity against human SW780 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SW780 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SW837	GI₅₀	33 nM Compound: GSK923295	Anticancer activity against human SW837 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SW837 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SW872	GI₅₀	37 nM Compound: GSK923295	Anticancer activity against human SW872 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SW872 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SW948	GI₅₀	98 nM Compound: GSK923295	Anticancer activity against human SW948 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SW948 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
SiHa	GI₅₀	29 nM Compound: GSK923295	Anticancer activity against human SiHa cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human SiHa cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
T-24	GI₅₀	45 nM Compound: GSK923295	Anticancer activity against human T24 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human T24 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
T47D	GI₅₀	29 nM Compound: GSK923295	Anticancer activity against human T47D cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human T47D cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
T84	GI₅₀	75 nM Compound: GSK923295	Anticancer activity against human T84 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human T84 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
THP-1	GI₅₀	12 nM Compound: GSK923295	Anticancer activity against human THP-1 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human THP-1 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
TK-10	GI₅₀	49 nM Compound: GSK923295	Anticancer activity against human TK-10 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human TK-10 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
U-251	GI₅₀	26 nM Compound: GSK923295	Anticancer activity against human U-251 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human U-251 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
U2OS	GI₅₀	45 nM Compound: GSK923295	Anticancer activity against human U2OS cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human U2OS cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
UACC-257	GI₅₀	32 nM Compound: GSK923295	Anticancer activity against human UACC-257 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human UACC-257 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
UACC-62	GI₅₀	25 nM Compound: GSK923295	Anticancer activity against human UACC-62 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human UACC-62 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
UMUC3	GI₅₀	23 nM Compound: GSK923295	Anticancer activity against human UM-UC-3 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human UM-UC-3 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
WiDr	GI₅₀	18 nM Compound: GSK923295	Anticancer activity against human WiDr cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human WiDr cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
Y79	GI₅₀	70 nM Compound: GSK923295	Anticancer activity against human Y79 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human Y79 cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]
YAPC	GI₅₀	59 nM Compound: GSK923295	Anticancer activity against human YAPC cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis Anticancer activity against human YAPC cells assessed as cell growth inhibition measured after 72 hrs by DAPI staining based analysis	[PMID: 20167803]

In Vitro

GSK-923295 (GSK923295) is a first-in-class, specific, allosteric inhibitor of CENP-E kinesin motor function. GSK923295 is uncompetitive with both ATP and MT, inhibiting CENP-E MT-stimulated ATPase activity with a K_i of 3.2±0.2 nM and 1.6±0.1 nM for human and canine, respectively. GSK923295 inhibits release of inorganic phosphate and stabilized CENP-E motor domain interaction with microtubules^[1]. GSK923295 has broad growth inhibitory activity in a panel of 237 cancer cell lines and produces significant tumor growth-delay in 8 of the 11 mouse xenograft tumor models with IC₅₀s of 17.2 nM, 55.6 nM, 42 nM, and 51.9 nM for SW48, RKO (BRAF mutant), SW620 (KRAS mutant), and HCT116 (KRAS mutant), respectively^[2]. GSK923295 is a potent and selective small molecule inhibitor of human CENPE with a K_i of 3.2 nM. GSK923295 demonstrates broad efficacy against a panel of 19 human neuroblastoma derived cell lines with an average growth IC₅₀ of 41 nM^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Xenografts of mice treated with GSK-923295 (GSK923295) shows significant tumor growth delay compared to the control arm (NB-EBc1 p<0.0001; NB-1643 p=0.018; NB-1691 p=0.0018)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

592.13

Formula

C₃₂H₃₈ClN₅O₄

CAS No.

1088965-37-0

Appearance

Solid

Color

White to yellow

SMILES

O=C(N[C@@H](CC1=CC=C(C2=CN3C(C([C@@H](O)C)=CC=C3)=N2)C=C1)CNC(CN(C)C)=O)C4=CC(Cl)=C(C=C4)OC(C)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 60 mg/mL (101.33 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6888 mL	8.4441 mL	16.8882 mL
5 mM	0.3378 mL	1.6888 mL	3.3776 mL
10 mM	0.1689 mL	0.8444 mL	1.6888 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.22 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.22 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.22 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.95%

Select Batch:

Data Sheet (279 KB) SDS (393 KB)

COA (249 KB) HNMR (148 KB) RP-HPLC (170 KB) NP-HPLC (158 KB) LCMS (106 KB)

Handling Instructions (2659 KB)

References

[1]. Wood KW, et al. Antitumor activity of an allosteric inhibitor of centromere-associated protein-E. Proc Natl Acad Sci U S A. 2010 Mar 30;107(13):5839-44. [Content Brief]

[2]. Mayes PA, et al. Mitogen-activated protein kinase (MEK/ERK) inhibition sensitizes cancer cells to centromere-associated protein E (CENP-E) inhibition. Int J Cancer. 2013 Feb 1;132(3):E149-57. [Content Brief]

[3]. Balamuth NJ, et al. Serial transcriptome analysis and cross-species integration identifies centromere-associated protein E as a novel neuroblastoma target. Cancer Res. 2010 Apr 1;70(7):2749-58. [Content Brief]

Cell Assay ^[1]	Cell-growth inhibition assays are performed by MDS in 384-well plates, and DNA content of fixed cells stained with DAPI using an Incell 1000 (GE) is analyzed. DNA content is determined 24 h after seeding (T₀) and after exposure to varying concentrations of GSK-923295 (0.01 nM, 0.1 nM, 1 nM, 10 nM, 100 nM, 1 μM, 10 μM, and 100 μM) for an additional 72 h (T₇₂). All T₇₂ measurements are normalized to T₀. Curves are analyzed using the XLfit curve-fitting tool to determine the concentration of GSK923295 yielding 50% growth inhibition relative to T₀ and Y_max values (GI₅₀)^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[3]	Mice^[3] CB17 scid mice are used to propagate subcutaneously implanted neuroblastoma tumors. Tumor diameters are measured using calipers. Tumor volumes are calculated. Once tumor volume exceeds 200 mm³, mice are randomized (n=10 per arm) to receive either GSK923295 125 mg/kg IP or vehicle (96% acidified water, 2% DMAC, 2% CREM) for a total of 6 doses using a 3 days on, 4 days off, 3 days on regimen. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Wood KW, et al. Antitumor activity of an allosteric inhibitor of centromere-associated protein-E. Proc Natl Acad Sci U S A. 2010 Mar 30;107(13):5839-44. [Content Brief] [2]. Mayes PA, et al. Mitogen-activated protein kinase (MEK/ERK) inhibition sensitizes cancer cells to centromere-associated protein E (CENP-E) inhibition. Int J Cancer. 2013 Feb 1;132(3):E149-57. [Content Brief] [3]. Balamuth NJ, et al. Serial transcriptome analysis and cross-species integration identifies centromere-associated protein E as a novel neuroblastoma target. Cancer Res. 2010 Apr 1;70(7):2749-58. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.6888 mL	8.4441 mL	16.8882 mL	42.2205 mL
	5 mM	0.3378 mL	1.6888 mL	3.3776 mL	8.4441 mL
	10 mM	0.1689 mL	0.8444 mL	1.6888 mL	4.2220 mL
	15 mM	0.1126 mL	0.5629 mL	1.1259 mL	2.8147 mL
	20 mM	0.0844 mL	0.4222 mL	0.8444 mL	2.1110 mL
	25 mM	0.0676 mL	0.3378 mL	0.6755 mL	1.6888 mL
	30 mM	0.0563 mL	0.2815 mL	0.5629 mL	1.4073 mL
	40 mM	0.0422 mL	0.2111 mL	0.4222 mL	1.0555 mL
	50 mM	0.0338 mL	0.1689 mL	0.3378 mL	0.8444 mL
	60 mM	0.0281 mL	0.1407 mL	0.2815 mL	0.7037 mL
	80 mM	0.0211 mL	0.1056 mL	0.2111 mL	0.5278 mL
	100 mM	0.0169 mL	0.0844 mL	0.1689 mL	0.4222 mL

GSK-923295 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

GSK-9232951088965-37-0GSK923295GSK 923295KinesinApoptosisInhibitorinhibitorinhibit

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