Kinesin-13

Kinesin-13 proteins are specialized microtubule (MT) depolymerases that regulate MT length by promoting tubulin removal at both ends^[1]^[2]. Unlike conventional motile kinesins, Kinesin-13 motors form ring and spiral oligomers at MT ends, which stabilizes their position for efficient depolymerization^[1]^[3]. Mechanistically, the conserved neck and loop-2 regions of Kinesin-13 coordinate ATP turnover to induce tubulin curvature and facilitate protofilament disassembly^[3]^[4]^[5]. This depolymerization is critical for spindle assembly, kinetochore-MT attachment, and accurate chromosome segregation during mitosis and meiosis^[2]^[6]^[7]. In disease models, Kinesin-13 dysregulation alters spindle morphology and microtubule flux, influencing cell division fidelity and axon regeneration^[8]^[9]^[10]. Compared with related isoforms such as Kinesin-8, Kinesin-13 exhibits higher end-specific depolymerization activity and distinct structural elements, including the α4-helix and KVD motif, which are essential for MT-end recognition^[11]^[12]^[13]. Kinesin-13 inhibitors and mutants that disrupt neck-mediated depolymerization or ATPase activity allow precise experimental modulation of MT dynamics for cell division and neuronal studies^[14]^[7]. Collectively, the Kinesin-13 family serves as a versatile model for understanding controlled MT depolymerization, isoform-specific regulation, and targeted cytoskeletal interventions in diverse eukaryotic systems^[15]^[16].

References:

[1]. Tan D, et al. Kinesin-13s form rings around microtubules. J Cell Biol. 2006 Oct 9;175(1):25-31. [Content Brief]

[2]. Ems-McClung SC, et al. Kinesin-13s in mitosis: Key players in the spatial and temporal organization of spindle microtubules. Semin Cell Dev Biol. 2010 May;21(3):276-82. [Content Brief]

[3]. Leong SY, et al. Kinesin-13 and Kinesin-8 Function during Cell Growth and Division in the Moss Physcomitrella patens. Plant Cell. 2020 Mar;32(3):683-702. [Content Brief]

[4]. Moores CA, et al. The role of the kinesin-13 neck in microtubule depolymerization. Cell Cycle. 2006 Aug;5(16):1812-5. [Content Brief]

[5]. Wickstead B, et al. The expanded Kinesin-13 repertoire of trypanosomes contains only one mitotic Kinesin indicating multiple extra-nuclear roles. PLoS One. 2010 Nov 23;5(11):e15020. [Content Brief]

[6]. Mulder AM, et al. A new model for binding of kinesin 13 to curved microtubule protofilaments. J Cell Biol. 2009 Apr 6;185(1):51-7. [Content Brief]

[7]. Belsham HR, et al. A synthetic ancestral kinesin-13 depolymerizes microtubules faster than any natural depolymerizing kinesin. Open Biol. 2022 Aug;12(8):220133. [Content Brief]

[8]. Hu H, et al. A kinesin-13 family kinesin in Trypanosoma brucei regulates cytokinesis and cytoskeleton morphogenesis by promoting microtubule bundling. PLoS Pathog. 2024 Feb 1;20(2):e1012000. [Content Brief]

[9]. Wagenbach M, et al. A kinesin-13 mutant catalytically depolymerizes microtubules in ADP. J Cell Biol. 2008 Nov 17;183(4):617-23. [Content Brief]

[10]. Friel CT, et al. The kinesin-13 MCAK has an unconventional ATPase cycle adapted for microtubule depolymerization. EMBO J. 2011 Aug 26;30(19):3928-39. [Content Brief]

[11]. Patel JT, et al. The family-specific α4-helix of the kinesin-13, MCAK, is critical to microtubule end recognition. Open Biol. 2016 Oct;6(10):160223. [Content Brief]

[12]. Do KK, et al. The kinesin-13 KLP10A motor regulates oocyte spindle length and affects EB1 binding without altering microtubule growth rates. Biol Open. 2014 Jun 6;3(7):561-70. [Content Brief]

[13]. Ghosh-Roy A, et al. Kinesin-13 and tubulin posttranslational modifications regulate microtubule growth in axon regeneration. Dev Cell. 2012 Oct 16;23(4):716-28. [Content Brief]

[14]. Wang W, et al. Insight into microtubule disassembly by kinesin-13s from the structure of Kif2C bound to tubulin. Nat Commun. 2017 Jul 10;8(1):70. [Content Brief]

[15]. Wang H, et al. Patronin mediates a switch from kinesin-13-dependent poleward flux to anaphase B spindle elongation. J Cell Biol. 2013 Oct 14;203(1):35-46. [Content Brief]

Kinesin-13 Related Products (2):

By Type:	Pan (1)
By Effects:	Inhibitor (1) Activator (1)

Cat. No.	Product Name	Effect	Purity

HY-122236

UMK57	Activator	99.51%
UMK57 is a MCAK activator and kinetochore-microtubule destabilizer. UMK57 enhances MCAK-dependent microtubule depolymerization, increases kinetochore-microtubule turnover, reduces chromosome mis-segregation and lagging chromosomes, and inhibits cancer cell proliferation. UMK57 triggers adaptive resistance in Aurora B cancer cells via reversible Aurora B signaling pathway alterations. UMK57 can be used for the research of solid tumors.

HY-177898

DHTP	Inhibitor	99.42%
DHTP is a kinesin-13 family of microtubule depolymerases selective allosteric inhibitor that inhibits kinesin-13 ATPase activity and microtubule depolymerization activity. DHTP inhibits Kif2a and MCAK with IC₅₀ values of 1.2 μM and 4.6 μM, respectively, and does not inhibit other kinesin families. DHTP modulates microtubule dynamics in cells, leading to less dynamic microtubules. DHTP links kinesin-13 overexpression to cancer and Paclitaxel (HY-B0015) resistance. DHTP can be used for the research of cancer.

Name
Email *

	Sorry, but the email address you supplied was invalid.