1. Academic Validation
  2. Mps1 controls spindle assembly, SAC, and DNA repair in the first cleavage of mouse early embryos

Mps1 controls spindle assembly, SAC, and DNA repair in the first cleavage of mouse early embryos

  • J Cell Biochem. 2021 Feb;122(2):290-300. doi: 10.1002/jcb.29858.
Jia-Qian Ju 1 Xiao-Han Li 1 Meng-Hao Pan 1 Yi Xu 1 Yao Xu 1 Ming-Hong Sun 1 Shao-Chen Sun 1
Affiliations

Affiliation

  • 1 College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
Abstract

Monopolar spindle-1 (Mps1) is a critical interphase regulator that also involves into the spindle assembly checkpoint for the cell cycle control in both mitosis and meiosis. However, the functions of Mps1 during mouse early embryo development is still unclear. In this study, we reported the important roles of Mps1 in the first cleavage of mouse embryos. Our data indicated that the loss of Mps1 activity caused precocious cleavage of zygotes to 2-cell embryos; however, prolonged culture disturbed the early embryo development to the blastocyst. We found that the spindle organization was disrupted after Mps1 inhibition, and the chromosomes were misaligned in the first cleavage. Moreover, the kinetochore-microtubule attachment was lost and Aurora B failed to accumulate to the kinetochores, indicating that the spindle assembly checkpoint (SAC) was activated. Furthermore, the inhibition of Mps1 activity resulted in an increase of DNA damage, which further induced oxidative stress, showing with positive γ-H2A.X signal and increased Reactive Oxygen Species level. Ultimately, irreparable DNA damage and oxidative stress-activated Apoptosis and Autophagy, which was confirmed by the positive Annexin-V signal and increased autophagosomes. Taken together, our data indicated that Mps1 played important roles in the control of SAC and DNA repair during mouse early embryo development.

Keywords

DNA damage; Mps1; embryo; spindle assembly checkpoint.

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Products
  • Cat. No.
    Product Name
    Description
    Target
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  • HY-13298
    99.37%, Mps1 Inhibitor