2. Academic Validation
  3. Cross and multiple herbicide resistance in annual bluegrass (Poa annua) populations from eastern Texas golf courses

Cross and multiple herbicide resistance in annual bluegrass (Poa annua) populations from eastern Texas golf courses

  • Pest Manag Sci. 2021 Apr;77(4):1903-1914. doi: 10.1002/ps.6217.
Vijay Singh 1 2,  Fabricia C Dos Reis 1 3,  Casey Reynolds 1 4,  Matthew Elmore 5 6,  Muthukumar Bagavathiannan 1
Affiliations

Affiliations

  • 1 Department of Soil and Crop Sciences, Texas A&M University, College Station, TX, USA.
  • 2 Eastern Shore Agricultural Research and Extension Center, Virginia Tech, Painter, VA, USA.
  • 3 Laboratório de Sementes e Mudas, Secretaria de Agricultura e Abastecimento, Campinas, Brazil.
  • 4 Turfgrass Producers International, Lombard, IL, USA.
  • 5 Texas A&M AgriLife Research and Extension Center, Dallas, TX, USA.
  • 6 Department of Plant Biology, Rutgers, The State University of New Jersey, New Brunswick, NJ, USA.
PMID: 33284481 DOI: 10.1002/ps.6217
Abstract

Background: Annual bluegrass is a troublesome weed in managed turf systems. A survey was conducted to evaluate the prevalence of Herbicide resistance in golf course populations of annual bluegrass in eastern Texas. Screenings were conducted for two Photosystem II (PS II)-inhibitor herbicides [simazine preemergence (PRE), amicarbazone postemergence (POST)], two acetolactate synthase (ALS) inhibitors (foramsulfuron POST, trifloxysulfuron POST) and one microtubule assembly inhibitor (pronamide PRE/POST).

Results: Ninety percent of the populations were found to be resistant to at least one of the tested herbicides. The TX15-14 population was >490-, 178-, 10-, 26-, 4.3- and 3.8-fold, and the TX15-27 population was >490-, 16-, 28-, 84-, 5.2- and 4.1-fold less sensitive to simazine, amicarbazone, foramsulfuron, trifloxysulfuron, pronamide POST and pronamide PRE, respectively, compared to the susceptible standard TX15-SUS. Populations resistant to pronamide POST were completely controlled by pronamide PRE at the label recommended rate. The ALS and psbA gene sequence analysis indicated the presence of target site mutations (Ser-264-Gly in the psbA gene of TX15-14 and Trp-574-Leu in the ALS gene of TX15-27). However, given the absence of any target-site mutation in the ALS gene of TX15-14, the psbA gene of TX15-27 and α-tubulin of both populations, nontarget site mechanisms of resistance are suspected.

Conclusion: This is the first case of multiple Herbicide resistance in annual bluegrass populations to three Herbicide modes of action. Results show the widespread occurrence of multiple Herbicide resistance in golf course annual bluegrass populations in eastern Texas and emphasize the need for the development and implementation of effective resistance management practices. © 2020 Society of Chemical Industry.

Keywords

ALS; PSII; herbicide resistance; microtubule assembly; resistance mechanism; target-site mutations; turfgrass.

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