1. Academic Validation
  2. Biochemical Properties of a Novel D-Mannose Isomerase from Pseudomonas syringae for D-Mannose Production

Biochemical Properties of a Novel D-Mannose Isomerase from Pseudomonas syringae for D-Mannose Production

  • Appl Biochem Biotechnol. 2021 May;193(5):1482-1495. doi: 10.1007/s12010-021-03487-y.
Xiaohan Hua # 1 Yanxiao Li # 2 Zhengqiang Jiang 1 Junwen Ma 2 Haijie Liu 1 Qiaojuan Yan 3
Affiliations

Affiliations

  • 1 Key Laboratory of Food Bioengineering (China National Light Industry), College of Food Science and Nutritional Engineering, China Agricultural University, No.17 Qinghua Donglu, Haidian District, Beijing, 100083, China.
  • 2 Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Engineering, China Agricultural University, No.17 Qinghua Donglu, Haidian District, Beijing, 100083, China.
  • 3 Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Engineering, China Agricultural University, No.17 Qinghua Donglu, Haidian District, Beijing, 100083, China. [email protected].
  • # Contributed equally.
Abstract

D-Mannose isomerase can reversibly catalyze D-fructose to D-mannose which has various beneficial effects. A novel D-mannose isomerase gene (PsMIaseA) from Pseudomonas syringae was cloned and expressed in Escherichia coli. The recombinant D-mannose isomerase (PsMIaseA) showed the highest amino acid sequence homogeneity of 50% with ManI from Thermobifda fusca. PsMIaseA was purified through Ni-NTA chromatography, and its specific activity was 818.6 U mg-1. The optimal pH and temperature of PsMIaseA were pH 7.5 and 45 °C, respectively. The Enzyme was stable within a wide pH range from 5.0 to 10.0. It could efficiently convert D-fructose to D-mannose without any metal ions. When PsMIaseA was incubated with 600 g/L D-fructose for 6 h, the space-time yield of D-mannose reached 27.2 g L-1 h-1 with a maximum conversion ratio of 27%. Therefore, the D-mannose isomerase may be suitable for green production of D-mannose.

Keywords

Characterization; Mannose; Mannose isomerase; Mannose production; Pseudomonas syringae.

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