2. Academic Validation
  3. Chemical Composition and Cytotoxic Activity of the Fractionated Trunk Bark Essential Oil from Tetraclinis articulata (Vahl) Mast. Growing in Tunisia

Chemical Composition and Cytotoxic Activity of the Fractionated Trunk Bark Essential Oil from Tetraclinis articulata (Vahl) Mast. Growing in Tunisia

  • Molecules. 2021 Feb 19;26(4):1110. doi: 10.3390/molecules26041110.
Salma Jlizi 1,  Aida Lahmar 2,  Afifa Zardi-Bergaoui 1,  Roberta Ascrizzi 3,  Guido Flamini 3 4,  Abdel Halim Harrath 5,  Leila Chekir-Ghedira 2,  Hichem Ben Jannet 1
Affiliations

Affiliations

  • 1 Laboratory of Heterocyclic Chemistry, Natural Products and Reactivity (LR11ES39), Medicinal Chemistry and Natural Products Team, Faculty of Science of Monastir, University of Monastir, Avenue of Environment, 5019 Monastir, Tunisia.
  • 2 Bioactive Natural Products and Biotechnology Research UnitUR17ES49, Faculty of Dental Medicine of Monastir, University of Monastir, Avicenne Street, 5000 Monastir, Tunisia.
  • 3 Dipartimento di Farmacia, Università di Pisa, Via Bonanno 6, 56126 Pisa, Italy.
  • 4 Centro Interdipartimentale di Ricerca "Nutraceutica e Alimentazione per la Salute", Università di Pisa, Via del Borghetto 80, 56124 Pisa, Italy.
  • 5 Department of Zoology, College of Science, King Saud University, Riyadh 11564, Saudi Arabia.
PMID: 33669825 DOI: 10.3390/molecules26041110
Abstract

The aim of the present research was to determine the chemical composition and the cytotoxic effects of Tetraclinis articulata trunk bark essential oil (HEE) obtained by steam distillation and five fractions obtained by normal phase silica chromatographic separation. Chemical analysis allowed the identification of 54 known compounds. Relatively high amounts of oxygenated Sesquiterpenes (44.4-70.2%) were detected, mainly consisting of caryophyllene oxide (13.1-26.6%), carotol (9.2-21.2%),14-hydroxy-9-epi-(E)-caryophyllene (3.2-15.5%) and humulene epoxide II (2.6-7.2%). The cytotoxic activity against human mammary carcinoma cell lines (MDA-MB-231) and colorectal carcinoma cell lines (SW620) of the essential oil and its fractions were assessed. All the samples displayed moderate to weak activity compared to 5-fluorouracil. The colorectal carcinoma cell line was relatively more sensitive to the essential oil and its fractions compared to the breast Cancer cell line, showing IC50 values from 25.7 to 96.5 μg/mL. In addition, the essential oil and its fraction E.2 revealed a cytotoxic activity against colorectal carcinoma cell line, with IC50 values lower than 30 μg/mL. This is the first report on the chemical composition and cytotoxic activity of the trunk bark essential oil of T. articulata.

Keywords

Tetraclinis articulata; chemical composition; cytotoxic activity; essential oil; fractionation; trunk bark.

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