1. Academic Validation
  2. Enhanced Eradication of Bacterial/Fungi Biofilms by Glucose Oxidase-Modified Magnetic Nanoparticles as a Potential Treatment for Persistent Endodontic Infections

Enhanced Eradication of Bacterial/Fungi Biofilms by Glucose Oxidase-Modified Magnetic Nanoparticles as a Potential Treatment for Persistent Endodontic Infections

  • ACS Appl Mater Interfaces. 2021 Apr 21;13(15):17289-17299. doi: 10.1021/acsami.1c01748.
Yanjing Ji 1 2 Zeyu Han 2 Han Ding 3 Xinkai Xu 2 Danyang Wang 1 2 3 Yanli Zhu 1 2 Fei An 2 Shang Tang 1 2 Hui Zhang 1 2 Jing Deng 1 2 Qihui Zhou 1 2 3
Affiliations

Affiliations

  • 1 Department of Stomatology, The Affiliated Hospital of Qingdao University, Qingdao 266003, China.
  • 2 School of Stomatology, Qingdao University, Qingdao 266003, China.
  • 3 Institute for Translational Medicine, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao 266021, China.
Abstract

Bacterial/Fungal biofilm-mediated persistent endodontic infections (PEIs) are one of the most frequent clinical lesions in the oral cavity, resulting in apical periodontitis and tooth damage caused by loss of Minerals. The conventional root canal disinfectants are poorly bio-safe and harmful to teeth and tissues, making them ineffective in treating PEIs. The development of nanomaterials is emerging as a promising strategy to eradicate disease-related bacteria/fungi. Herein, glucose oxidase (GOx)-modified magnetic nanoparticles (MNPs) were synthesized via a facile and versatile route for investigating their effects on removing PEI-related Bacterial/Fungal biofilms. It is found that GOx was successfully immobilized on the MNPs by detecting the changes in the diameter, chemical functional group, charge, and magnetic response. Further, we demonstrate that GOx-modified MNPs (GMNPs) exhibit highly effective Antibacterial activity against Enterococcus faecalis and Candida albicans. Moreover, the Antibacterial/Fungal activity of GMNPs is greatly dependent on their concentrations. Importantly, when placed in contact with Bacterial/Fungal biofilms, the dense biofilm matrix is destructed due to the movement of GMNPs induced by the magnetic field, the formation of Reactive Oxygen Species, and nutrient starvation induced by GOx. Also, the in vitro experiment shows that the as-prepared GMNPs have excellent cytocompatibility and blood compatibility. Thus, GMNPs offer a novel strategy to treat bacteria/fungi-associated PEIs for potential clinical applications.

Keywords

bacterial/fungi biofilms; glucose oxidase; magnetic nanoparticles; persistent endodontic infections; reactive oxygen species; root canal disinfectant.

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