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  3. Mir-34c affects the proliferation and pluripotency of porcine induced pluripotent stem cell (piPSC)-like cells by targeting c-Myc

Mir-34c affects the proliferation and pluripotency of porcine induced pluripotent stem cell (piPSC)-like cells by targeting c-Myc

  • Cells Dev. 2021 Jun:166:203665. doi: 10.1016/j.cdev.2021.203665.
Wei Yue 1,  Jing Sun 1,  Juqing Zhang 1,  Yongxing Chang 1,  Qiaoyan Shen 1,  Zhenshuo Zhu 1,  Shuai Yu 1,  Xiaolong Wu 1,  Sha Peng 1,  Na Li 1,  Jinlian Hua 2
Affiliations

Affiliations

  • 1 College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
  • 2 College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: [email protected].
PMID: 33994350 DOI: 10.1016/j.cdev.2021.203665
Abstract

MicroRNAs are important regulators in stem cells, which involve in gene regulation, including cell proliferation, differentiation and Apoptosis. As an important one, miR-34c participates in various processes by targeting protein-coding genes. It is generally considered as a tumor suppressor and cell adhesion inhibitor. However, whether miR-34c has effects on pluripotent stem cells is not clear. Here, by mir-34c mimics transfection, the function of miR-34c on porcine induced pluripotent stem cell (piPSC)-like cells was investigated. Bioinformatics analyses showed that c-Myc is miR-34c's candidate target, which was confirmed by dual Luciferase assay. The knockout of miR-34c indicated that mir-34c affects the proliferation and pluripotency of piPSC-like cells by targeting c-Myc. Our study explored the regulatory mechanism of miR-34c on piPSC-like cells, providing a reference for the establishment of true porcine PSCs.

Keywords

Cell proliferation; Pluripotency; c-Myc; miR-34c; piPSC-like cells.

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