2. Academic Validation
  3. Effect of Fucoxanthinol on Pancreatic Ductal Adenocarcinoma Cells from an N-Nitrosobis(2-oxopropyl)amine-initiated Syrian Golden Hamster Pancreatic Carcinogenesis Model

Effect of Fucoxanthinol on Pancreatic Ductal Adenocarcinoma Cells from an N-Nitrosobis(2-oxopropyl)amine-initiated Syrian Golden Hamster Pancreatic Carcinogenesis Model

  • Cancer Genomics Proteomics. 2021 May-Jun;18(3 Suppl):407-423. doi: 10.21873/cgp.20268.
Masaru Terasaki 1 2,  Yusaku Nishizaka 3,  Wataru Murase 3,  Atsuhito Kubota 3,  Hiroyuki Kojima 3 2,  Mareshige Kojoma 3,  Takuji Tanaka 4,  Hayato Maeda 5,  Kazuo Miyashita 6,  Michihiro Mutoh 7,  Mami Takahashi 8
Affiliations

Affiliations

  • 1 School of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Hokkaido, Japan; [email protected].
  • 2 Advanced Research Promotion Center, Health Sciences University of Hokkaido, Hokkaido, Japan.
  • 3 School of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Hokkaido, Japan.
  • 4 Department of Diagnostic Pathology and Research Center of Diagnostic Pathology, Gifu Municipal Hospital, Gifu, Japan.
  • 5 Faculty of Agriculture and Life Science, Hirosaki University, Aomori, Japan.
  • 6 Center for Industry-University Collaboration, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.
  • 7 Department of Molecular-Targeting Prevention, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan.
  • 8 Central Animal Division, National Cancer Center, Tokyo, Japan.
PMID: 33994364 DOI: 10.21873/cgp.20268
Abstract

Background/aim: Fucoxanthinol (FxOH) is a marine carotenoid metabolite with potent anti-cancer activity. However, little is known about the efficacy of FxOH in pancreatic Cancer. In the present study, we investigated the inhibitory effect of FxOH on six types of cells cloned from N-nitrosobis(2-oxopropyl)amine (BOP)-induced hamster pancreatic Cancer (HaPC) cells.

Materials and methods: FxOH action and its molecular mechanisms were investigated in HaPC cells using flow-cytometry, comprehensive gene array, and western blotting analyses.

Results: FxOH (5.0 μM) significantly suppressed the growth of four out of six types of HaPC cells. Moreover, FxOH significantly suppressed cell cycle, chemokine, Integrin, actin polymerization, microtubule organization and PI3K/Akt and TGF-β signals, and activated Caspase-3 followed by Apoptosis and anoikis induction in HaPC-5 cells.

Conclusion: FxOH may have a high potential as a Cancer chemopreventive agent in a hamster pancreatic carcinogenesis model.

Keywords

Apoptosis; CXCR7; carotenoid; fucoxanthinol; hamster pancreatic cancer.

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