2. Academic Validation
  3. Protocol for microglia replacement by peripheral blood (Mr PB)

Protocol for microglia replacement by peripheral blood (Mr PB)

  • STAR Protoc. 2021 Jun 11;2(2):100613. doi: 10.1016/j.xpro.2021.100613.
Zhen Xu 1,  Yanxia Rao 2,  Bo Peng 3
Affiliations

Affiliations

  • 1 Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
  • 2 Shanghai Key Laboratory of Psychotic Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 201108, China.
  • 3 Department of Neurosurgery, Huashan Hospital, Institute for Translational Brain Research, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Fudan University, Shanghai 200032, China.
PMID: 34179837 DOI: 10.1016/j.xpro.2021.100613
Abstract

Microglia are important immune cells in the central nervous system. Replacement of mutated microglia by wild-type cells through microglia replacement by bone marrow transplantation can correct gene deficiencies. However, the limited availability of bone marrow cells may restrict its potential of becoming a widely used clinical treatment. Here, we introduce a potentially clinical-feasible strategy achieving efficient microglia replacement by peripheral blood cells in mice, boosting the donor cell availability. We named it microglia replacement by peripheral blood (Mr PB). For complete details on the use and execution of this protocol, please refer to Xu et al. (2020). The original abbreviation of this microglia replacement strategy is mrPB. We hereby change the name to Mr PB.

Keywords

Cell Biology; Model Organisms; Neuroscience.

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