2. Academic Validation
  3. Microglia replacement by bone marrow transplantation (Mr BMT) in the central nervous system of adult mice

Microglia replacement by bone marrow transplantation (Mr BMT) in the central nervous system of adult mice

  • STAR Protoc. 2021 Jul 7;2(3):100666. doi: 10.1016/j.xpro.2021.100666.
Zhen Xu 1,  Xin Zhou 2,  Bo Peng 2,  Yanxia Rao 3
Affiliations

Affiliations

  • 1 Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
  • 2 Department of Neurosurgery, Huashan Hospital, Institute for Translational Brain Research, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Fudan University, Shanghai 200032, China.
  • 3 Shanghai Key Laboratory of Psychotic Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 201108, China.
PMID: 34286294 DOI: 10.1016/j.xpro.2021.100666
Abstract

Microglia are important immune cells in the central nervous system (CNS). Mutations in microglia may cause CNS disorders. Replacement of dysfunctional microglia with allogeneic wild-type microglia can correct the gene deficiency, thus treating the neurogenic diseases. However, traditional approaches cannot efficiently replace microglia at the adulthood. Here, we introduce a potentially clinical-feasible strategy named microglia replacement by bone marrow transplantation that achieves efficient microglia replacement at the whole CNS scale, including the brain, spinal cord, and retina in adult mice. For complete details on the use and execution of this protocol, please refer to Xu et al. (2020). The original abbreviation of this microglia replacement strategy is mrBMT. We hereby change the name to Mr BMT.

Keywords

Cell Biology; Immunology; Microscopy; Model Organisms; Neuroscience; Stem Cells.

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