2. Academic Validation
  3. Microglia replacement by microglia transplantation (Mr MT) in the adult mouse brain

Microglia replacement by microglia transplantation (Mr MT) in the adult mouse brain

  • STAR Protoc. 2021 Jul 9;2(3):100665. doi: 10.1016/j.xpro.2021.100665.
Zhen Xu 1,  Bo Peng 2,  Yanxia Rao 3
Affiliations

Affiliations

  • 1 Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
  • 2 Department of Neurosurgery, Huashan Hospital, Institute for Translational Brain Research, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Fudan University, Shanghai 200032, China.
  • 3 Shanghai Key Laboratory of Psychotic Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 201108, China.
PMID: 34308380 DOI: 10.1016/j.xpro.2021.100665
Abstract

Mutations in microglia may cause brain disorders. Replacement of dysfunctional microglia by allogeneic wild-type microglia from bone marrow transplantation (Mr BMT) or peripheral blood can correct the gene deficiency at the brain-wide scale but cannot achieve precise replacement at specific brain regions. Here, we introduce a strategy with potential clinical relevance-microglia replacement by microglia transplantation (Mr MT), combining tamoxifen-induced ablation of Mr BMT cells and intracranial injection of microglia to mouse brain, to achieve region-sepcific microglia replacement. The original abbreviation of this microglia replacement strategy is mrMT. We hereby change the name to Mr MT. For complete details on the use and execution of this protocol, please refer to Xu et al. (2020).

Keywords

Cell Biology; Immunology; Microscopy; Model Organisms; Neuroscience; Stem Cells.

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