2. Academic Validation
  3. Purification, Physicochemical Properties, and Antioxidant Activities of Two Low-Molecular-Weight Polysaccharides from Ganoderma leucocontextum Fruiting Bodies

Purification, Physicochemical Properties, and Antioxidant Activities of Two Low-Molecular-Weight Polysaccharides from Ganoderma leucocontextum Fruiting Bodies

  • Antioxidants (Basel). 2021 Jul 20;10(7):1145. doi: 10.3390/antiox10071145.
Xiong Gao 1 2,  Jiayi Qi 3,  Chi-Tang Ho 4,  Bin Li 3 5,  Yizhen Xie 1 2,  Shaodan Chen 1,  Huiping Hu 1,  Zhongzheng Chen 3 5,  Qingping Wu 1
Affiliations

Affiliations

  • 1 State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Safety and Health, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.
  • 2 Guangdong Yuewei Edible Fungi Technology Co. Ltd., Guangzhou 510663, China.
  • 3 Department of Bioengineering, College of Food Science, South China Agricultural University, 483 Wushan Street, Tianhe District, Guangzhou 510642, China.
  • 4 Department of Food Science, Rutgers University, 65 Dudley Road, New Brunswick, NJ 08901, USA.
  • 5 Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, Department of Food Science, College of Food Science, South China Agricultural University, Guangzhou 510642, China.
PMID: 34356378 DOI: 10.3390/antiox10071145
Abstract

Two low-molecular-weight Polysaccharides (GLP-1 and GLP-2) were purified from Ganoderma leucocontextum fruiting bodies, and their physicochemical properties and antioxidant activities were investigated and compared in this study. The results showed that GLP-1 and GLP-2 were mainly composed of mannose, glucose, galactose, xylose, and arabinose, with weight-average molecular weights of 6.31 and 14.07 kDa, respectively. Additionally, GLP-1 and GLP-2 had a similar chain conformation, crystal structure, and molecular surface morphology. Moreover, GLP-1 exhibited stronger antioxidant activities than GLP-2 in five different assays: 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), hydroxyl radical, superoxide anion radical, ferric reducing antioxidant power (FRAP), and oxygen radical antioxidant capacity (ORAC). The main linkage types of GLP-1 were found to be →4)-α-D-Glcp-(1→, →4)-β-D-Glcp-(1→, →3)-β-D-Glcp-(1→, →6)-β-D-Galp-(1→, →6)-α-D-Glcp-(1→, →4,6)-α-D-Glcp-(1→, and Glcp-(1→ by methylation analysis and nuclear magnetic resonance (NMR) spectroscopy. In addition, GLP-1 could protect NIH3T3 cells against tert-butyl hydroperoxide (tBHP)-induced oxidative damage by increasing catalase (CAT) and Glutathione Peroxidase (GSH-Px) activities, elevating the glutathione/oxidized glutathione (GSH/GSSG) ratio, and decreasing the malondialdehyde (MDA) level. These findings indicated that GLP-1 could be explored as a potential antioxidant agent for application in functional foods.

Keywords

Ganoderma leucocontextum; antioxidant activity; physicochemical property; polysaccharides.

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