2. Academic Validation
  3. The polypeptide OP3-4 induced osteogenic differentiation of bone marrow mesenchymal stem cells via protein kinase B/glycogen synthase kinase 3β/β-catenin pathway and promoted mandibular defect bone regeneration

The polypeptide OP3-4 induced osteogenic differentiation of bone marrow mesenchymal stem cells via protein kinase B/glycogen synthase kinase 3β/β-catenin pathway and promoted mandibular defect bone regeneration

  • Arch Oral Biol. 2021 Oct:130:105243. doi: 10.1016/j.archoralbio.2021.105243.
Congshan Li 1,  Panpan Yang 1,  Yuying Kou 1,  Dong Zhang 2,  Minqi Li 3
Affiliations

Affiliations

  • 1 Center of Osteoporosis and Bone Mineral Research, Shandong University, Jinan, China; Department of Bone Metabolism, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, China.
  • 2 Department of Oral and Maxillofacial Surgery, Qilu Hospital of Shandong University, Jinan, China. Electronic address: [email protected].
  • 3 Center of Osteoporosis and Bone Mineral Research, Shandong University, Jinan, China; Department of Bone Metabolism, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, China. Electronic address: [email protected].
PMID: 34416564 DOI: 10.1016/j.archoralbio.2021.105243
Abstract

Objectives: The aims of this study were to explore: (ⅰ) the effect of the polypeptide OP 3-4 on bone regeneration in vivo; (ⅱ) the effect of OP 3-4 on osteogenic differentiation of bone marrow mesenchymal stem cells in vitro; and (ⅲ) the potential mechanism of OP 3-4 in promoting osteogenic differentiation of bone marrow mesenchymal stem cells.

Designs: 30 Wistar rats (8-week, male) were randomly divided into Control group (n = 5), Hydrogel group (n = 5), and Hydrogel loaded OP 3-4 group (n = 5). Hematoxylin and eosin staining was used to evaluate the level of bone regeneration in mandibular defect. Immunohistochemistry staining was used to evaluate the expression of Alkaline Phosphatase, runt-related transcription factor 2, and type Ⅰ Collagen. Flow cytometry was applied to identify the phenotype of bone marrow mesenchymal stem cells. Furthermore, LY294002, the inhibitor of protein kinase B, was applied to verify the role of OP 3-4 in promoting osteogenic differentiation via protein kinase B/glycogen synthase kinase 3β/β-catenin pathway through western blot.

Results: OP 3-4 promoted bone regeneration of rat mandibular defect. The expression of osteogenic differentiation related markers were increased after adding OP 3-4 to bone marrow mesenchymal stem cells. OP 3-4 promoted osteogenic differentiation of bone marrow mesenchymal stem cells via protein kinase B/glycogen synthase kinase 3β/β-catenin pathway.

Conclusion: OP 3-4 could promote bone regeneration of mandibular defect and improve osteogenic differentiation through protein kinase B/glycogen synthase kinase 3β/β-catenin pathway.

Keywords

Bone marrow mesenchymal stem cells; Bone regeneration; Mandibular defect; OP3-4; Osteogenic differentiation; Protein kinase B/glycogen synthase kinase 3β/β-catenin pathway.

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