1. Academic Validation
  2. Biological Effect of Differently Sized Tetrahedral Framework Nucleic Acids: Endocytosis, Proliferation, Migration, and Biodistribution

Biological Effect of Differently Sized Tetrahedral Framework Nucleic Acids: Endocytosis, Proliferation, Migration, and Biodistribution

  • ACS Appl Mater Interfaces. 2021 Dec 8;13(48):57067-57074. doi: 10.1021/acsami.1c20657.
Sirong Shi 1 Yanjing Li 1 2 Tao Zhang 1 Dexuan Xiao 1 Taoran Tian 1 Tianyu Chen 1 Yun Zhang 1 Xiaobing Li 1 3 Yunfeng Lin 1 4
Affiliations

Affiliations

  • 1 State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
  • 2 Tianjin Medical University School of Stomatology, Tianjin 300203, China.
  • 3 State Key Laboratory of Oral Diseases, Department of Pediatric Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, PR China.
  • 4 College of Biomedical Engineering, Sichuan University, Chengdu 610041, China.
Abstract

With the advent of nanotechnology, DNA nanostructures have been widely applied in various fields, particularly biology and biomedicine. Tetrahedral framework nucleic acids (TFNAs), a novel type of DNA nanomaterial, have attracted considerable attention due to their simple synthesis, high accessibility, structural stability, and versatility. However, to date, the interaction of differently sized TFNAs with living systems and their ability to be endocytosed and biodistributed in mouse is still not fully understood. To screen for the optimal TFNA size and structures, TFNA endocytosis, proliferation, and migration were tested in adipose stem cells (ASCs). We found that the internalization of differently sized TFNAs in ASCs was remarkably different. Although all TFNAs could enter ASCs, T21 had the best membrane-penetrating ability. After exposure of ASCs to TFNAs of different sizes, the proliferation and migration of cells were enhanced, especially with T21. Importantly, T21 could access the brain and accumulate over time. This study improves our understanding of the influence of TFNA size on the biological behavior of ASCs, which will help in choosing optimal TFNA size for biomedical applications.

Keywords

and migration; different sizes; endocytosis; proliferation; tetrahedral framework nucleic acids (TFNAs).

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