2. Academic Validation
  3. Marker-based assays for studying gut transit in gnotobiotic and conventional mouse models

Marker-based assays for studying gut transit in gnotobiotic and conventional mouse models

  • STAR Protoc. 2021 Nov 5;2(4):100938. doi: 10.1016/j.xpro.2021.100938.
Sean T Koester 1,  Naisi Li 1,  Daniel M Lachance 1 2,  Neelendu Dey 1 3 4
Affiliations

Affiliations

  • 1 Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
  • 2 Molecular Engineering & Sciences Institute, University of Washington, Seattle, WA 98105, USA.
  • 3 Microbiome Research Initiative, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
  • 4 Department of Medicine, Division of Gastroenterology, University of Washington, Seattle, WA 98195, USA.
PMID: 34806048 DOI: 10.1016/j.xpro.2021.100938
Abstract

Gastrointestinal motility is regulated by a variety of environmental factors including gut microbes and metabolites. The ability to interrogate mouse models of gut motility has enabled elucidation of these relationships. Here we describe integration of the red carmine dye and fluorescence isothiocyanate-dextran marker-based assays for characterizing gut transit with spatial resolution, along with an optional intracolonic infusion protocol for studying the effects of metabolites on colonic transit. These protocols can be adapted for use in gnotobiotic and conventional mouse models. For complete details on the use and execution of this protocol, please refer to Li et al. (2021).

Keywords

Microbiology; Model Organisms.

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