2. GPCR/G Protein
  3. G protein-coupled Bile Acid Receptor 1
  4. SBI-115

SBI-115 is a TGR5 (GPCR19) antagonist. SBI-115 decreases hepatic cystogenesis with polycystic liver diseases via inhibiting TGR5.

For research use only. We do not sell to patients.

CAS No. : 882366-16-7

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10 mM * 1 mL in DMSO
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Customer Review

Based on 35 publication(s) in Google Scholar

SBI-115 Related Antibodies

Top Publications Citing Use of Products

35 Publications Citing Use of MCE SBI-115

WB
RT-PCR
Flow Cytometry
Cell Migration/Invasion Assay

    SBI-115 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Oct 14;13(1):6081.  [Abstract]

    SBI-115 (10 μM, 48 h) inhibited 1,5-AG-induced expression of collagen I and vimentin in primary mouse renal tubular epithelial cells.

    SBI-115 purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2022 Feb;12(2):787-800.

    SBI-115 (10 μM, 48 h) decreased the upregulation of M2 markers, accompanied by increased expression of the M1 marker, in BMDMs that were cultured with CM collected from LLC cell culture.

    SBI-115 purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2022 Feb;12(2):787-800.

    SBI-115 (5 or 10 μM, 48 h) resulted in increased tumor phagocytic capacity of CM-treated BMDMs.

    SBI-115 purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2022 Feb;12(2):787-800.

    SBI-115 (5 or 10 μM, 48 h) inhibited CM-macrophage-induced Lewis lung carcinoma cell migration and invasion.

    SBI-115 purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2022 Feb;12(2):787-800.

    SBI-115 (5 or 10 μM, 48 h) increased the percentage of granzyme B⁺ cells among CD8b⁺ T cells co-cultured with Lewis lung carcinoma CM-educated BMDMs.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    SBI-115 is a TGR5 (GPCR19) antagonist. SBI-115 decreases hepatic cystogenesis with polycystic liver diseases via inhibiting TGR5[1].

    In Vitro

    SBI-115 (100-200 μM, 24 hours) inhibits proliferation triggered by pre-treatment of cystic cholangiocytes with Taurolithocholic acid (TLCA) in shRNA-transfected ADPKD cholangiocytes[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    SBI-115 Related Antibodies

    Cell Proliferation Assay[1]

    Cell Line: shRNA-transfected ADPKD cholangiocytes
    Concentration: 100, 200 µM
    Incubation Time: 24 hours
    Result: Inhibited proliferation (by 32-48%) triggered by pre-treatment of cystic cholangiocytes with TLCA.
    Molecular Weight

    340.78

    Formula

    C14H13ClN2O4S
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(C1=NC(S(=O)(CC)=O)=NC=C1Cl)OC2=CC=CC(C)=C2
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (293.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.9344 mL 14.6722 mL 29.3444 mL
    5 mM 0.5869 mL 2.9344 mL 5.8689 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  2% DMSO    40% PEG300    5% Tween-80    53% Saline

      Solubility: 3 mg/mL (8.80 mM); Suspended solution; Need ultrasonic

    • Protocol 2

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (7.34 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  Corn Oil

      Solubility: 5 mg/mL (14.67 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.58%

    SDS (393 KB)

    COA (248 KB) HNMR (135 KB) RP-HPLC (270 KB) LCMS (386 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.9344 mL 14.6722 mL 29.3444 mL 73.3611 mL
    5 mM 0.5869 mL 2.9344 mL 5.8689 mL 14.6722 mL
    10 mM 0.2934 mL 1.4672 mL 2.9344 mL 7.3361 mL
    15 mM 0.1956 mL 0.9781 mL 1.9563 mL 4.8907 mL
    20 mM 0.1467 mL 0.7336 mL 1.4672 mL 3.6681 mL
    25 mM 0.1174 mL 0.5869 mL 1.1738 mL 2.9344 mL
    30 mM 0.0978 mL 0.4891 mL 0.9781 mL 2.4454 mL
    40 mM 0.0734 mL 0.3668 mL 0.7336 mL 1.8340 mL
    50 mM 0.0587 mL 0.2934 mL 0.5869 mL 1.4672 mL
    60 mM 0.0489 mL 0.2445 mL 0.4891 mL 1.2227 mL
    80 mM 0.0367 mL 0.1834 mL 0.3668 mL 0.9170 mL
    100 mM 0.0293 mL 0.1467 mL 0.2934 mL 0.7336 mL
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    SBI-115 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    SBI-115882366-16-7SBI115SBI 115G protein-coupled Bile Acid Receptor 1G-protein coupled receptor 19GPCR19TGR5GPBAR1Inhibitorinhibitorinhibit

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