1. Academic Validation
  2. Overexpression of N-acetylglucosaminyltransferase V promotes human parotid gland acinar cell immortalization via the epidermal receptor activation

Overexpression of N-acetylglucosaminyltransferase V promotes human parotid gland acinar cell immortalization via the epidermal receptor activation

  • J Cell Physiol. 2022 Mar;237(3):1780-1789. doi: 10.1002/jcp.30641.
Ching-Chia Cheng 1 Chih-Feng Lin 2 Yong-Chong Lin 1 Tai-Horng Young 1 3 Pei-Jen Lou 2
Affiliations

Affiliations

  • 1 Department of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan.
  • 2 Department of Otolaryngology, National Taiwan University Hospital and College of Medicine, Taipei, Taiwan.
  • 3 Department of Biomedical Engineering, National Taiwan University Hospital, Taipei, Taiwan.
Abstract

The purpose of this study is to maintain the proliferation capability of human parotid gland acinar cells (ACs) in vitro to extend passage number and to study the mechanism that regulates AC stemness. N-acetylglucosaminyltransferase V (GnT-V) is the Golgi Enzyme, and it has been reported that the β1,6GlcNAc-branched N-linked glycans are associated with various cell behaviors. Therefore, we modify the gene expression of ACs by transfection of the GnT-V-overexpression plasmid, and we found that upregulation of GnT-V extensively increased ACs proliferation and stemness properties in ACs/GnT-V compared to ACs transfected with Mock plasmid. More importantly, we observed that high levels of GnT-V positively correlated with ALDH1A3 expression via increasing phosphorylation of cell surface receptors and activating the downstream signaling transduction. Hence, the current study suggested that GnT-V is a significant factor for cell immortalization in the ACs model by activating the EGFR/ERK/ALDH1A3 signaling pathway.

Keywords

ALDH1 enzyme; EGF receptor; MAP-ERK kinase; N-acetylglucosamine transferases; acini cells.

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