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  3. Anisotropic magneto-mechanical stimulation on collagen coatings to accelerate osteogenesis

Anisotropic magneto-mechanical stimulation on collagen coatings to accelerate osteogenesis

  • Colloids Surf B Biointerfaces. 2022 Feb:210:112227. doi: 10.1016/j.colsurfb.2021.112227.
Suya Lin 1,  Juan Li 2,  Jiaqi Shao 2,  Jiamin Zhang 1,  Xuzhao He 1,  Donghua Huang 3,  Lingqing Dong 4,  Jun Lin 5,  Wenjian Weng 1,  Kui Cheng 6
Affiliations

Affiliations

  • 1 School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Center of Rehabilitation Biomedical Materials, Cyrus Tang Center for Sensor Materials and Applications, Zhejiang University, Hangzhou 310027, China.
  • 2 The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China.
  • 3 Department of Orthopaedic Surgery, the Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China.
  • 4 The Affiliated Stomatologic Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China.
  • 5 The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China. Electronic address: [email protected].
  • 6 School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Center of Rehabilitation Biomedical Materials, Cyrus Tang Center for Sensor Materials and Applications, Zhejiang University, Hangzhou 310027, China; Department of Rehabilitation Medicine, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China. Electronic address: [email protected].
PMID: 34838419 DOI: 10.1016/j.colsurfb.2021.112227
Abstract

Mechanical stimulation has been considered to be critical to cellular response and tissue regeneration. However, harnessing the direction of mechanical stimulation during osteogenesis still remains a challenge. In this study, we designed a series of novel magnetized Collagen coatings (MCCs) (randomly or parallel-oriented Collagen fibers) to exert the anisotropic mechanical stimulation using oriented magnetic actuation during osteogenesis. Strikingly, we found the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) were significantly up-regulated when the direction of magnetic actuation was parallel to the randomly-oriented Collagen coating surface, in contrast to the down-regulated capacity under the perpendicular magnetic actuation. Moreover, further exerting a parallel mechanical stimulation along the parallel-oriented Collagen coating, which cells have been oriented by the oriented collagens, were not only able to up-regulate the osteogenic differentiation of BMSCs but also promote the new bone formation during osteogenesis in vivo. We also demonstrated the anisotropic magneto-mechanical stimulation for the osteogenic differences might be attributed to the stretching or bending tensile status of Collagen fibers controlled by the direction of magnetic actuation, driving the α5β1-dependent Integrin signaling cascade. This study therefore got insight of understanding the directional mechanical stimulation on osteogenesis, and also paved a way for sustaining regulation of the biomaterials-host interface.

Keywords

BMSCs; Magnetic actuation direction; Magnetized collagen coatings; Mechanical stimulation; Osteogenic differentiation.

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