2. Academic Validation
  3. GKK1032B from endophytic Penicillium citrinum induces the apoptosis of human osteosarcoma MG63 cells through caspase pathway activation

GKK1032B from endophytic Penicillium citrinum induces the apoptosis of human osteosarcoma MG63 cells through caspase pathway activation

  • Chin J Nat Med. 2022 Jan;20(1):67-73. doi: 10.1016/S1875-5364(21)60108-5.
Na Liu 1,  Mei-Na Song 1,  Qian-Qian Zhang 1,  Cong Wu 1,  Kong-Kai Zhu 1,  Yu-Lin Sun 1,  Meng-Ru Li 1,  Feng-Ying Yang 1,  Run-Liang Feng 1,  Yu-Ying Zhang 2,  Hua Zhang 3
Affiliations

Affiliations

  • 1 School of Biological Science and Technology, University of Jinan, Jinan 250022, China.
  • 2 School of Biological Science and Technology, University of Jinan, Jinan 250022, China. Electronic address: [email protected].
  • 3 School of Biological Science and Technology, University of Jinan, Jinan 250022, China. Electronic address: [email protected].
PMID: 35101251 DOI: 10.1016/S1875-5364(21)60108-5
Abstract

Chemical investigation of the culture extract of an endophytic Penicillium citrinum from Dendrobium officinale, afforded nine citrinin derivatives (1-9) and one peptide-polyketide hybrid GKK1032B (10). The structures of these compounds were determined by spectroscopic methods. The absolute configurations of 1 and 2 were determined for the first time by calculation of electronic circular dichroism (ECD) data. Among them, GKK1032B (10) showed significant cytotoxicity against human osteosarcoma cell line MG63 with an IC50 value of 3.49 μmol·L-1, and a primary mechanistic study revealed that it induced the Apoptosis of MG63 cellsvia Caspase pathway activation.

Keywords

Anti-proliferation; Apoptosis; Endophytic; GKK1032B; Penicillium citrinum.

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