2. Academic Validation
  3. Viridiflorol induces anti-neoplastic effects on breast, lung, and brain cancer cells through apoptosis

Viridiflorol induces anti-neoplastic effects on breast, lung, and brain cancer cells through apoptosis

  • Saudi J Biol Sci. 2022 Feb;29(2):816-821. doi: 10.1016/j.sjbs.2021.10.026.
Maaged A Akiel 1 2,  Ohoud Y Alshehri 3,  Shokran A Aljihani 3,  Amani Almuaysib 4,  Ammar Bader 5,  Ahmed I Al-Asmari 6,  Hassan S Alamri 1,  Bahauddeen M Alrfaei 4,  Majed A Halwani 3
Affiliations

Affiliations

  • 1 Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud Bin Abdulaziz University for Health Sciences (KSAU-HS), King Abdullah International Medical Research Center (KAIMRC), Riyadh, Saudi Arabia.
  • 2 Department of Human and Molecular Genetics, Virginia Commonwealth University, Richmond, VA, United States.
  • 3 Nanomedicine Department, King Abdullah International Medical Research Center/ King Abdulaziz Medical City, Ministry of National Guard, Health Affairs, Riyadh, Saudi Arabia.
  • 4 Stem Cells and Regenerative Medicine, King Abdullah International Medical Research Center/ King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.
  • 5 Faculty of Pharmacy, Umm Al-Qura University, Makkah 21955, Saudi Arabia.
  • 6 King Abdul-Aziz Hospital, Laboratory Department, Ministry of Health, Jeddah, Saudi Arabia.
PMID: 35197749 DOI: 10.1016/j.sjbs.2021.10.026
Abstract

All active natural molecules are not fully exploited as therapeutic agents, causing delays in the advancement of Anticancer drug discovery. Viridiflorol is a natural volatile element that may work as anti-cancer compound. We tested the Anticancer properties of viridiflorol at different concentrations ranging from 0.03 to 300 μM in vitro on three Cancer cells including breast (MCF-7), lung (A549) and brain (Daoy). The Cancer cells responses were documented after treatment using MTT and Annexin V assays. Viridiflorol showed cytotoxic effects against all tested cell lines, reducing cell viability in a concentration-dependent manner with variable IC50 values. Daoy and A549 cell lines were more sensitive to viridiflorol when compared with temozolomide and doxorubicin, respectively. Viridiflorol demonstrated the highest Anticancer activity against the Daoy cells with an estimated IC50 of 0.1 µM followed by MCF-7 at 10 µM, and A549 at 30 µM. In addition, upon exposure to concentrations ranging from 30 µM to 300 µM of viridiflorol, early and late apoptotic cell death was induced in a concentration dependent manner in Daoy (55.8%-72.1%), MCF-7 (36.2%-72.7%) and A459 (35%-98.9%) cell lines, respectively. In conclusion, viridiflorol demonstrates cytotoxic and apoptotic ability in three different Cancer cell lines (brain, breast and lung).

Keywords

Anticancer; Apoptosis; Natural product; Viridiflorol, Cytotoxicity, Drug discovery.

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