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  3. In Vivo Aniline Blue Staining and Semiautomated Quantification of Callose Deposition at Plasmodesmata

In Vivo Aniline Blue Staining and Semiautomated Quantification of Callose Deposition at Plasmodesmata

  • Methods Mol Biol. 2022:2457:151-165. doi: 10.1007/978-1-0716-2132-5_9.
Caiping Huang 1,  Jerôme Mutterer 1,  Manfred Heinlein 2
Affiliations

Affiliations

  • 1 Institut de Biologie Moléculaire des Plantes, CNRS, Université de Strasbourg, Strasbourg, France.
  • 2 Institut de Biologie Moléculaire des Plantes, CNRS, Université de Strasbourg, Strasbourg, France. [email protected].
PMID: 35349138 DOI: 10.1007/978-1-0716-2132-5_9
Abstract

The deposition and turnover of callose (beta-1,3 glucan polymer) in the cell wall surrounding the neck regions of plasmodesmata (PD) controls the cell-to-cell diffusion rate of molecules and, therefore, plays an important role in the regulation of intercellular communication in Plants.Here we describe a simple and fast in vivo staining procedure for the imaging and quantification of callose at PD. We also introduce calloseQuant, a plug-in for semiautomated image analysis and non-biased quantification of callose levels at PD using ImageJ.

Keywords

Aniline blue; Callose; Confocal microscopy; ImageJ; Plasmodesmata; flg22.

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