Aniline blue diammonium 

Aniline blue diammonium is a component of commonly used polychrome stains. Aniline blue diammonium is used to stain collagen fibers in tissue sections using Masson′s trichrome protocol for staining multiple components. Collagen is stained blue by this method. The dye is suitable for selective staining of callose in plant specimens and staining histones for assessing nuclear maturity. Aniline blue diammonium is used in Gomori′s one-step trichrome stain and Mallory′s connective tissue stain for tissue including kidney and intestine.

789.90

C₃₇H₃₅N₅O₉S₃

66687-07-8

O=S(C1=CC=C(C=C1)/N=C2C=C/C(C=C\2)=C(C3=CC=C(C=C3)NC4=CC=C(C=C4)S(O)(=O)=O)\C5=CC=C(C=C5)NC6=CC=C(C=C6)S(=O)(O)=O)(O)=O.N.N

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Huang C, Mutterer J, Heinlein M. In Vivo Aniline Blue Staining and Semiautomated Quantification of Callose Deposition at Plasmodesmata. Methods Mol Biol. 2022;2457:151-165.  [Content Brief]

  [2]. Gou LT, Lim DH, Ma W, et al. Initiation of Parental Genome Reprogramming in Fertilized Oocyte by Splicing Kinase SRPK1-Catalyzed Protamine Phosphorylation. Cell. 2020;180(6):1212-1227.e14.  [Content Brief]