1. Academic Validation
  2. Biointerface topography mediates the interplay between endothelial cells and monocytes

Biointerface topography mediates the interplay between endothelial cells and monocytes

  • RSC Adv. 2020 Apr 6;10(23):13848-13854. doi: 10.1039/d0ra00704h.
Yan Liu 1 Wenshuai Deng 2 Liangliang Yang 3 Xiuxiu Fu 4 Zhibin Wang 4 Patrick van Rijn 3 Qihui Zhou 1 5 Tao Yu 1 4
Affiliations

Affiliations

  • 1 Institute for Translational Medicine, School of Basic Medicine, Qingdao University Qingdao 266021 China [email protected] [email protected].
  • 2 Department of Neurosurgery, The Affiliated Hospital of Qingdao University Qingdao 266003 China.
  • 3 University of Groningen, W. J. Kolff Institute for Biomedical Engineering and Materials Science, Department of Biomedical Engineering, University Medical Center Groningen A. Deusinglaan 1 9713 AV Groningen the Netherlands [email protected].
  • 4 Department of Echocardiography, The Affiliated Hospital of Qingdao University Qingdao 266003 China.
  • 5 Stomatology Center, The Affiliated Hospital of Qingdao University Qingdao 266003 China.
Abstract

Endothelial cell (EC) monolayers located in the inner lining of blood vessels serve as a semipermeable barrier between circulating blood and surrounding tissues. The structure and function of the EC monolayer affect the recruitment and adhesion of monocytes, which plays a pivotal role in the development of inflammation and atherosclerosis. Here we investigate the effect of material wrinkled topographies on the responses of human umbilical vein endothelial cells (HUVECs) and adhesion of monocytes to HUVECs. It is found that HUVEC responses are non-linearly mediated by surface topographies with different dimensions. Specifically, more cell elongation and better cell orientation on the wrinkled surface with a 3.5 μm amplitude and 10 μm wavelength (W10) are observed compared to other surfaces. The proliferation rate of HUVECs on the W10 surface is higher than that on other surfaces due to more 5-ethynyl-2'-deoxyuridine (EdU) detected on the W10 surface. Also, greater expression of inflammatory cytokines from HUVECs and adhesion of monocytes to HUVECs on the W10 surface is shown than other surfaces due to greater expression of p-AKT and ICAM, respectively. This study offers a new in vitro system to understand the interplay between HUVEC monolayers and monocytes mediated by aligned topographies, which may be useful for vascular repair and disease modeling for drug testing.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-D0938
    99.01%, Cell Proliferation Fluorescent Probe