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  3. Isolation and comparison of neural stem cells from the adult rat brain and spinal cord canonical neurogenic niches

Isolation and comparison of neural stem cells from the adult rat brain and spinal cord canonical neurogenic niches

  • STAR Protoc. 2022 Jun 7;3(2):101426. doi: 10.1016/j.xpro.2022.101426.
Lars Erik Schiro 1,  Ulrich Stefan Bauer 1,  Axel Sandvig 1 2 3,  Ioanna Sandvig 1
Affiliations

Affiliations

  • 1 Department of Neuromedicine and Movement Science, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology (NTNU), 7030 Trondheim, Norway.
  • 2 Department of Clinical Neuroscience, Division of Neuro, Head, and Neck, Umeå University Hospital, 907 37 Umeå, Sweden.
  • 3 Department of Community and Rehabilitation, Division of Neuro, Head and Neck, Umeå University Hospital, 907 37 Umeå, Sweden.
PMID: 35693206 DOI: 10.1016/j.xpro.2022.101426
Abstract

Here, we present a unified protocol for the extraction, culture, and basic characterization of rat neural stem cells (NSCs) from all three canonical neurogenic niches in the brain and spinal cord. We describe tissue dissection and dissociation, Cell Culture, followed by EdU labeling and characterization of NSCs. By yielding considerable numbers of viable cells per animal, this protocol enables the establishment of substantial, long-term cell banks, thus offering cost and labor efficiency while significantly reducing the numbers of Animals used.

Keywords

Cell Biology; Cell culture; Cell isolation; Microscopy; Model Organisms; Neuroscience; Stem Cells.

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