2. Academic Validation
  3. Extracellular matrix derived from Wharton's Jelly-derived mesenchymal stem cells promotes angiogenesis via integrin αVβ3/c-Myc/P300/VEGF

Extracellular matrix derived from Wharton's Jelly-derived mesenchymal stem cells promotes angiogenesis via integrin αVβ3/c-Myc/P300/VEGF

  • Stem Cell Res Ther. 2022 Jul 18;13(1):327. doi: 10.1186/s13287-022-03009-5.
Beilei Ma 1,  Tengkai Wang 2,  Juan Li 3,  Qian Wang 4
Affiliations

Affiliations

  • 1 Department of Clinical Laboratory, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, 250012, Shandong, China.
  • 2 Department of Gastroenterology, Qilu Hospital of Shandong University, Jinan, 250012, Shandong, China.
  • 3 Department of Clinical Laboratory, Qilu Hospital of Shandong University (Qingdao), Qingdao, 266035, China.
  • 4 Department of Clinical Laboratory, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, 250012, Shandong, China. [email protected].
PMID: 35851415 DOI: 10.1186/s13287-022-03009-5
Abstract

Background: Angiogenesis is required in many physiological conditions, including bone regeneration, wound healing, and tissue regeneration. Mesenchymal stem cells-derived extracellular matrix (MSCs-ECM) could guide intricate cellular and tissue processes such as homeostasis, healing and regeneration.

Methods: The purpose of this study is to explore the effect and mechanism of ECM derived from decellularized Wharton's Jelly-derived mesenchymal stem cells (WJ-MSCs) on endothelial cell viability and angiogenesis. The human umbilical vein endothelial cells (HUVECs) were pretreated with WJ-MSCs ECM for 2d/7d/14d, respectively. After pretreatment, the angiogenesis ability of HUVECs was detected.

Results: In this study, we found for the first time that WJ-MSCs ECM could improve the angiogenesis ability of HUVECs with a time-dependent manner in vitro. Mechanically, WJ-MSCs ECM activated the focal adhesion kinase (FAK)/P38 signaling pathway via Integrin αVβ3, which further promoted the expression of the cellular (c)-Myc. Further, c-Myc increased histone acetylation levels of the vascular endothelial growth factor (VEGF) promoter by recruiting P300, which ultimately promoting VEGF expression.

Conclusions: ECM derived from Wharton's Jelly-derived mesenchymal stem cells promotes angiogenesis via Integrin αVβ3/c-Myc/P300/VEGF. This study is expected to provide a new approach to promote angiogenesis in bone and tissue regeneration.

Keywords

Angiogenesis; Histone acetylation level; Mesenchymal stem cells-derived extracellular matrix; Vascular endothelial growth factor; Wharton's Jelly-derived mesenchymal stem cells.

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