2. Academic Validation
  3. Acute hypoxia promotes the liver angiogenesis of largemouth bass (Micropterus salmoides) by HIF - Dependent pathway

Acute hypoxia promotes the liver angiogenesis of largemouth bass (Micropterus salmoides) by HIF - Dependent pathway

  • Fish Shellfish Immunol. 2022 Dec:131:264-273. doi: 10.1016/j.fsi.2022.08.007.
Liulan Zhao 1,  Xiaohong Tang 2,  Rui Huang 3,  Qiao Liu 4,  Lei Liao 5,  Yifan Hu 6,  Kuo He 7,  Xin Zhang 8,  Jiazhong Guo 9,  Shiyi Chen 10,  Song Yang 11
Affiliations

Affiliations

  • 1 College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. Electronic address: [email protected].
  • 2 College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China; Fish Resources and Environment in the Lpper Reaches of the Yangtze River Observation and Research Station of Sichuan Province, Chengdu, Sichuan, 610011, China. Electronic address: [email protected].
  • 3 College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. Electronic address: [email protected].
  • 4 College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. Electronic address: [email protected].
  • 5 College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. Electronic address: [email protected].
  • 6 College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. Electronic address: [email protected].
  • 7 College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. Electronic address: [email protected].
  • 8 College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. Electronic address: [email protected].
  • 9 College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. Electronic address: [email protected].
  • 10 College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. Electronic address: [email protected].
  • 11 College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. Electronic address: [email protected].
PMID: 35940542 DOI: 10.1016/j.fsi.2022.08.007
Abstract

A 24-h hypoxia exposure experiment was conducted to determine how hypoxia exposure induce liver angiogenesis in largemouth bass. Nitrogen (N2) was pumped into water to exclude dissolved oxygen into 1.2 ± 0.2 mg/L, and liver tissues were sampled during hypoxia exposure of 0 h, 4 h, 8 h, 12 h, 24 h and re-oxygenation for 12 h. Firstly, the results showed that hypoxia exposure promoted the angiogenesis occurrence by immunohistochemical analysis of vascular endothelial growth factor receptor 2 (VEGFR2). Secondly, the concentration of vasodilation factor increased and it's activity was elevated during 8 h exposure, such as nitric oxide (NO) and nitric oxide synthase (NOS) (p < 0.05). Thirdly, hypoxia exposure promoted angiogenesis through up-regulation the expression of matrix metalloproteinase 2 (MMP-2), jagged, protein kinase B (Akt), phosphoinositide-3-kinase (PI3K), mitogen-activated protein kinase (MAPK) at 4 h; contrarily, the expression of inhibiting angiogenesis genes presented up-regulated at 8 h (p < 0.05), such as matrix metalloproteinase inhibitor-2 (TIMP-2), matrix metalloproteinase inhibitor-3 (TIMP-3). Finally, the genes and proteins that regulate angiogenesis presented obvious chronological order. Parts of them promoted the budding and extension of blood vessels were up-regulated during 4 h-8 h (p < 0.05), such as vascular endothelial growth factor a (VEGFA), VEGFR2, monocarboxylic acid transporter 1 (MCT1), CD147, prolyl hydroxylase (PHD), nuclear factor kappa-B (NF-κB); Other part of them promoted blood vessel maturation were highly expressed during 12 h-24 h (p < 0.05), such as angiogenin-1 (ANG-1) and angiogenin-2 (ANG-2). In short, acute hypoxia can promote the liver angiogenesis of largemouth bass by HIF - dependent pathway.

Keywords

Angiogenesis; Hypoxia; Hypoxia inducible factor; Micropterus salmoides; Vascular endothelial growth factor.

Figures
Products