2. Academic Validation
  3. Protocol to derive human trophoblast stem cells directly from primed pluripotent stem cells

Protocol to derive human trophoblast stem cells directly from primed pluripotent stem cells

  • STAR Protoc. 2022 Aug 18;3(3):101638. doi: 10.1016/j.xpro.2022.101638.
Yanxing Wei 1,  Lu Xiao 2,  Lishi Ma 2,  Zhijian Wang 2,  Liping Huang 2,  Huiying Li 3,  Guangjin Pan 4,  Stephen J Lye 5,  Yongli Shan 6
Affiliations

Affiliations

  • 1 Placenta Research Group, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; Lunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, ON M5T3H7, Canada; Key Laboratory of Functional Proteomics of Guangdong Province, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China; The First Clinical Medical School, Southern Medical University, Guangzhou 510515, China.
  • 2 Placenta Research Group, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
  • 3 The First Clinical Medical School, Southern Medical University, Guangzhou 510515, China.
  • 4 CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
  • 5 Lunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, ON M5T3H7, Canada; Department of Obstetrics & Gynecology, University of Toronto, Toronto, ON M5G1L4, Canada; Department of Physiology, University of Toronto, Toronto, ON M5G1L4, Canada.
  • 6 Placenta Research Group, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China. Electronic address: [email protected].
PMID: 36042882 DOI: 10.1016/j.xpro.2022.101638
Abstract

Human trophoblast stem cells (hTSCs) are useful for studying human placenta development and diseases, but primed human pluripotent stem cells (hPSCs) routinely cultured in most laboratories do not support hTSC derivation. Here, we present a protocol to derive hTSCs directly from primed hPSCs. This approach, containing two strategies either with or without bone morphogenetic protein 4 (BMP4), provides a simple and accessible tool for deriving hTSCs to study placenta development and disease modeling without ethical limitations or reprogramming process. For complete details on the use and execution of this protocol, please refer to Wei et al. (2021).

Keywords

Cell biology; Cell culture; Cell differentiation; Developmental biology; Stem cells.

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