2. Academic Validation
  3. Study on the potential of Sanghuangporus sanghuang and its components as COVID-19 spike protein receptor binding domain inhibitors

Study on the potential of Sanghuangporus sanghuang and its components as COVID-19 spike protein receptor binding domain inhibitors

  • Biomed Pharmacother. 2022 Sep:153:113434. doi: 10.1016/j.biopha.2022.113434.
Liang-Hsuan Chien 1,  Jeng-Shyan Deng 2,  Wen-Ping Jiang 3,  Chin-Chu Chen 4,  Ya-Ni Chou 1,  Jaung-Geng Lin 5,  Guan-Jhong Huang 6
Affiliations

Affiliations

  • 1 Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Chinese Medicine, China Medical University, Taichung 404, Taiwan.
  • 2 Department of Food Nutrition and Healthy Biotechnology, Asia University,Taichung 413, Taiwan, Asia University, Taichung 413, Taiwan.
  • 3 Department of Pharmacy, Chia Nan University of Pharmacy and Science, Tainan 717, Taiwan.
  • 4 Grape King Biotechnology Inc., Chungli, Taiwan.
  • 5 Department of Chinese Medical, China Medical University, Taichung 404, Taiwan. Electronic address: [email protected].
  • 6 Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Chinese Medicine, China Medical University, Taichung 404, Taiwan; Department of Food Nutrition and Healthy Biotechnology, Asia University,Taichung 413, Taiwan, Asia University, Taichung 413, Taiwan. Electronic address: [email protected].
PMID: 36076488 DOI: 10.1016/j.biopha.2022.113434
Abstract

Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and has led to the most severe global pandemic, which began in Wuhan, China. Angiotensin-converting enzyme 2 (ACE2) combines with the spike protein of SARS-CoV-2, allowing the virus to cross the membrane and enter the cell. SARS-CoV-2 is modified by the transmembrane protease serine 2 (TMPRSS2) to facilitate access to cells. Accordingly, ACE2 and TMPRSS2 are targets of vital importance for the avoidance of SARS-CoV-2 Infection. Sanghuangporus sanghuang (SS) is a traditional Chinese medicine that has been demonstrated to have antitumor, antioxidant, anti-inflammatory, antidiabetic, hepatoprotective, neuroprotective and immunomodulatory properties. In this paper, we demonstrated that SS decreased ACE2 and TMPRSS2 expression in cell lines and a mouse model without cytotoxicity or organ damage. Liver and kidney sections were confirmed to have reduced expression of ACE2 and TMPRSS2 by immunohistochemistry (IHC) assessment. Then, hispidin, DBA, PAC, PAD and CA, phenolic compounds of SS, were also tested and verified to reduce the expression of ACE2 and TMPRSS2. In summary, the results indicate that SS and its phenolic compounds have latent capacity for preventing SARS-CoV-2 Infection in the future.

Keywords

3,4- dihydroxybenzalacetone; ACE2; Caffeic acid; Hispidin; Protocatechualdehyde; Protocatechuic acid; SARS-CoV-2; Sanghuangporus sanghuang; TMPRSS2.

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