2. Academic Validation
  3. Visualizing inflammation with an M1 macrophage selective probe via GLUT1 as the gating target

Visualizing inflammation with an M1 macrophage selective probe via GLUT1 as the gating target

  • Nat Commun. 2022 Oct 10;13(1):5974. doi: 10.1038/s41467-022-33526-z.
Heewon Cho # 1,  Haw-Young Kwon # 2,  Amit Sharma 3,  Sun Hyeok Lee 1,  Xiao Liu 4,  Naoki Miyamoto 2,  Jong-Jin Kim 5,  Sin-Hyeog Im 3 6 7,  Nam-Young Kang 8,  Young-Tae Chang 9 10 11
Affiliations

Affiliations

  • 1 School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang, 37673, Republic of Korea.
  • 2 Center for Self-assembly and Complexity, Institute for Basic Science (IBS), Pohang, 37673, Republic of Korea.
  • 3 Department of Life Sciences, Pohang University of Science and Technology (POSTECH), Pohang, 37673, Republic of Korea.
  • 4 Department of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang, 37673, Republic of Korea.
  • 5 Department of Biology, Sunchon National University, Sunchon, 57922, Republic of Korea.
  • 6 ImmunoBiome Inc., Pohang, 37666, Republic of Korea.
  • 7 Institute for Convergence Research and Education in Advanced Technology, Yonsei University, Seoul, 03722, Republic of Korea.
  • 8 Department of Convergence IT Engineering, Pohang University of Science and Technology (POSTECH), Pohang, 37673, Republic of Korea.
  • 9 School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang, 37673, Republic of Korea. [email protected].
  • 10 Center for Self-assembly and Complexity, Institute for Basic Science (IBS), Pohang, 37673, Republic of Korea. [email protected].
  • 11 Department of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang, 37673, Republic of Korea. [email protected].
  • # Contributed equally.
PMID: 36216803 DOI: 10.1038/s41467-022-33526-z
Abstract

Macrophages play crucial roles in protecting our bodies from Infection and cancers. As macrophages are multi-functional immune cells, they have diverse plastic subsets, such as M1 and M2, derived from naïve M0 cells. Subset-specific macrophage probes are essential for deciphering and monitoring the various activation of macrophages, but developing such probes has been challenging. Here we report a fluorescent probe, CDr17, which is selective for M1 macrophages over M2 or M0. The selective staining mechanism of CDr17 is explicated as Gating-Oriented Live-cell Distinction (GOLD) through overexpressed GLUT1 in M1 macrophages. Finally, we demonstrate the suitability of CDr17 to track M1 macrophages in vivo in a rheumatoid arthritis animal model.

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