2. Academic Validation
  3. Single Cell RNA-Seq Identifies Immune-Related Prognostic Model and Key Signature-SPP1 in Pancreatic Ductal Adenocarcinoma

Single Cell RNA-Seq Identifies Immune-Related Prognostic Model and Key Signature-SPP1 in Pancreatic Ductal Adenocarcinoma

  • Genes (Basel). 2022 Sep 29;13(10):1760. doi: 10.3390/genes13101760.
Kai Chen 1,  Qi Wang 1,  Xinxin Liu 1,  Feng Wang 2,  Yongsu Ma 1,  Shupeng Zhang 3,  Zhijiang Shao 3,  Yinmo Yang 1,  Xiaodong Tian 1
Affiliations

Affiliations

  • 1 Department of General Surgery, Peking University First Hospital, Beijing 100034, China.
  • 2 Department of Endoscopy Center, Peking University First Hospital, Beijing 100034, China.
  • 3 Department of General Surgery, Tianjin Fifth Centre Hospital, Tianjin 300450, China.
PMID: 36292645 DOI: 10.3390/genes13101760
Abstract

There are no reliable biomarkers for early diagnosis or prognosis evaluation in pancreatic ductal adenocarcinoma (PDAC). Multiple scRNA-seq datasets for PDAC were retrieved from online databases and combined with scRNA-seq results from our previous study. The malignant ductal cells were identified through calculating copy number variation (CNV) scores. The robust markers of malignant ductal cells in PDAC were found. Five immune-related signatures, including SPP1, LINC00683, SNHG10, LINC00237, and CASC19, were used to develop a risk score formula to predict the overall survival of PDAC patients. We also constructed an easy-to-use nomogram, combining risk score, N stage, and margin status. The expression level of SPP1 was related to the prognosis and immune regulators. We found that SPP1 was mainly expressed in ductal cells and macrophages in PDAC. In conclusion, we constructed a promising prognostic model based on immune-related signatures for PDAC using scRNA-seq and TCGA_PAAD datasets.

Keywords

SPP1; ScRNA-seq; immune related signatures; pancreatic cancer; prognostic model.

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