2. Academic Validation
  3. MicroRNA 101 Attenuated NSCLC Proliferation through IDH2/HIF α Axis Suppression in the Warburg Effect

MicroRNA 101 Attenuated NSCLC Proliferation through IDH2/HIF α Axis Suppression in the Warburg Effect

  • Oxid Med Cell Longev. 2022 Oct 18:2022:4938811. doi: 10.1155/2022/4938811.
Le Han 1 2,  Yili Zhang 1,  Bin Zhao 1,  Jianren Yue 1,  Zhenghong Chen 1,  Guangyan Lei 1,  Chen Huang 2,  Wenjuan Chen 1
Affiliations

Affiliations

  • 1 Cancer Hospital of Shaanxi Province, Affiliated to the Medical College of Xi'an Jiaotong University, Xi'an, 710061 Shaanxi, China.
  • 2 Department of Cell Biology and Genetics/Key Laboratory of Environment and Genes Related to Diseases, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, China.
PMID: 36304962 DOI: 10.1155/2022/4938811
Abstract

Lung Cancer is the most diagnosed and deadly Cancer in China. MicroRNAs are small noncoding RNA gene products that exhibit multifunctional regulation in Cancer cell progressions. MiR-101 loss was illustrated in about 29% of lung Cancer patients, and sophisticated mechanisms of miR-101 regulation in NSCLC are eager to be disclosed. Here, using specimens from NSCLC patients and Dural-luciferase reporter assay, we got a clue that miR-101 correlated with IDH2. MiR-101 overexpression and IDH2 deficiency both suppressed NSCLC tumor growth in mice. Moreover, in NSCLC, miR-101 suppressed IDH2 expression levels, further increased α-KG concentration, and finally inhibited the Warburg effect under hypoxic conditions through downregulating HIF1α expression by promoting HIF1α hydroxylation and degradation. In conclusion, miR-101 attenuated the Warburg effect and NSCLC proliferation through IDH2/HIF1α pathway.

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