2. Academic Validation
  3. Non-electric bioelectrical analog strategy by a biophysical-driven nano-micro spatial anisotropic scaffold for regulating stem cell niche and tissue regeneration in a neuronal therapy

Non-electric bioelectrical analog strategy by a biophysical-driven nano-micro spatial anisotropic scaffold for regulating stem cell niche and tissue regeneration in a neuronal therapy

  • Bioact Mater. 2022 Jun 13:20:319-338. doi: 10.1016/j.bioactmat.2022.05.034.
Xiangyun Yao 1 2 3,  Lei Zhan 4,  Zhiwen Yan 1 2 3,  Juehong Li 1 2 3,  Lingchi Kong 1 2 3,  Xu Wang 1 2 3,  Huimin Xiao 5,  Huiquan Jiang 5,  Chen Huang 4,  Yuanming Ouyang 1 2 3,  Yun Qian 1 2 3,  Cunyi Fan 1 2 3
Affiliations

Affiliations

  • 1 Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, China.
  • 2 Shanghai Engineering Research Center for Orthopaedic Material Innovation and Tissue Regeneration, Shanghai, 200233, China.
  • 3 Youth Science and Technology Innovation Studio of Shanghai Jiao Tong University School of Medicine, Shanghai, 200233, China.
  • 4 Key Laboratory of Textile Science and Technology, College of Textiles, Donghua University, Shanghai, 201620, China.
  • 5 College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China.
PMID: 36380746 DOI: 10.1016/j.bioactmat.2022.05.034
Abstract

The slow regenerating rate and misdirected axonal growth are primary concerns that disturb the curative outcome of peripheral nerve repair. Biophysical intervention through nerve scaffolds can provide efficient, tunable and sustainable guidance for nerve regrowth. Herein, we fabricate the reduced graphene oxide (rGO)/polycaprolactone (PCL) scaffold characterized with anisotropic microfibers and oriented nanogrooves by electrospinning technique. Adipose-derived stem cells (ADSCs) are seeded on the scaffolds in vitro and the viability, neural differentiation efficiency and neurotrophic potential are investigated. RGO/PCL conduits reprogram the phenotype of seeded cells and efficiently repair 15 mm sciatic nerve defect in rats. In summary, biophysical cues on nerve scaffolds are key determinants to stem cell phenotype, and ADSC-seeded rGO/PCL oriented scaffolds are promising, controllable and sustainable approaches to enable peripheral nerve regeneration.

Keywords

Adipose-derived stem cell; Bioelectrical; Biophysical; Nano-micro; Nerve regeneration; Reduced graphene oxide.

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