2. Academic Validation
  3. A Novel Role of the Two-Component System Response Regulator UvrY in Enterohemorrhagic Escherichia coli O157:H7 Pathogenicity Regulation

A Novel Role of the Two-Component System Response Regulator UvrY in Enterohemorrhagic Escherichia coli O157:H7 Pathogenicity Regulation

  • Int J Mol Sci. 2023 Jan 24;24(3):2297. doi: 10.3390/ijms24032297.
Pan Wu 1 2,  Qian Wang 1 2,  Qian Yang 1 2,  Xiaohui Feng 1 2,  Xingmei Liu 1 2,  Hongmin Sun 1 2,  Jun Yan 1 2,  Chenbo Kang 1 2,  Bin Liu 1 2 3,  Yutao Liu 1 2 3,  Bin Yang 1 2
Affiliations

Affiliations

  • 1 TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin 300457, China.
  • 2 Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin 300457, China.
  • 3 Nankai International Advanced Research Institute, Nankai University, Shenzhen 518000, China.
PMID: 36768620 DOI: 10.3390/ijms24032297
Abstract

Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is an important human pathogen causing severe diseases, such as hemorrhagic colitis and lethal hemolytic uremic syndrome. The signal-sensing capability of EHEC O157:H7 at specific host colonization sites via different two-component systems (TCSs) is closely related to its pathogenicity during Infection. However, the types of systems involved and the regulatory mechanisms are not fully understood. Here, we investigated the function of the TCS BarA/UvrY regulator UvrY in the pathogenicity regulation of EHEC O157:H7. Our results showed that UvrY acts as a positive regulator of EHEC O157:H7 for cellular adherence and mouse colonization through the transcriptional activation of the locus for enterocyte effacement (LEE) pathogenic genes. Furthermore, this regulation is mediated by the LEE island master regulator, Ler. Our results highlight the significance of UvrY in EHEC O157:H7 pathogenicity and underline the unknown importance of BarA/UvrY in colonization establishment and intestinal adaptability during Infection.

Keywords

LEE genes; UvrY; enterohemorrhagic Escherichia coli (EHEC) O157:H7; pathogenicity; two-component systems (TCSs).

Figures
Products