2. Academic Validation
  3. Curcumin-stabilized silver nanoparticles encapsulated in biocompatible electrospun nanofibrous scaffold for sustained eradication of drug-resistant bacteria

Curcumin-stabilized silver nanoparticles encapsulated in biocompatible electrospun nanofibrous scaffold for sustained eradication of drug-resistant bacteria

  • J Hazard Mater. 2023 Jun 15:452:131290. doi: 10.1016/j.jhazmat.2023.131290.
Ling Cai 1,  Xinyi Zhu 2,  Hongjie Ruan 3,  Jing Yang 2,  Wei Wei 4,  Yuan Wu 5,  Liuzhu Zhou 2,  Huijun Jiang 6,  Minghui Ji 7,  Jin Chen 8
Affiliations

Affiliations

  • 1 Center for Global Health, The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, China; School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China.
  • 2 Center for Global Health, The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, China.
  • 3 Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, 123 Tianfei Lane, Nanjing 210004, China.
  • 4 School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China.
  • 5 Department of Medical Oncology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing 210009, China.
  • 6 School of Pharmacy, Nanjing Medical University, Nanjing 211166, China.
  • 7 School of Nursing, Nanjing Medical University, Nanjing 211166, China.
  • 8 Center for Global Health, The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, China; School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China; Jiangsu Province Engineering Research Center of Antibody Drug, Key Laboratory of Antibody Technique of National Health Commission, Nanjing Medical University, Nanjing 211166, China. Electronic address: [email protected].
PMID: 37023575 DOI: 10.1016/j.jhazmat.2023.131290
Abstract

Due to the misuse of Antibiotics, the emerging drug-resistance of pathogenic microbes has aroused considerable concerns for the public health, which demands the continuous search for safe and efficient antimicrobial treatment. In this study, curcumin reduced and stabilized silver nanoparticles (C-Ag NPs) were successfully encapsulated into electrospun nanofiber membranes consisted of polyvinyl alcohol (PVA) cross-linked by citric acids (CA), which exhibited desirable biocompatibility and broad-spectrum antimicrobial activities. The homogeneously distributed and sustained release of C-Ag NPs in the constructed nanofibrous scaffolds yield prominent killing effect against Escherichia coli, Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus (MRSA), which involved the Reactive Oxygen Species (ROS) generation. Outstanding elimination of Bacterial biofilms and excellent Antifungal activity against Candida albicans was also identified after treated with PVA/CA/C-Ag. Transcriptomic analysis on MRSA treated by PVA/CA/C-Ag revealed the Antibacterial process is related to disrupting carbohydrate and energy metabolism, as well as destroying Bacterial membranes. Significant down-regulation of the expression of multidrug-resistant efflux pump gene sdrM was observed pointing to the role of PVA/CA/C-Ag to overcome the Bacterial resistance. Therefore, the constructed ecofriendly and biocompatible nanofibrous scaffolds provide a robust and versatile nanoplatform of reversal potential to eradicate drug-resistant pathogenic microbe in environmental as well as healthcare applications.

Keywords

Antibiotics-resistant bacteria; Antimicrobial; Electrospun nanofibrous membrane; Silver nanoparticles; Transcriptomic analysis.

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