1. Academic Validation
  2. High-dose synthetic phenolic antioxidant propyl gallate impairs mouse oocyte meiotic maturation through inducing mitochondrial dysfunction and DNA damage

High-dose synthetic phenolic antioxidant propyl gallate impairs mouse oocyte meiotic maturation through inducing mitochondrial dysfunction and DNA damage

  • Environ Toxicol. 2023 Apr 13. doi: 10.1002/tox.23807.
Sheng-Ji Yang 1 Yong-Sheng Wang 1 Li-Dan Zhang 1 Zhi-Ming Ding 1 Xu Zhou 1 Ze-Qun Duan 1 Ming Liu 1 Ai-Xin Liang 1 2 Li-Jun Huo 1 2
Affiliations

Affiliations

  • 1 Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China; College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, People's Republic of China.
  • 2 National Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Huazhong Agricultural University, Wuhan, People's Republic of China.
Abstract

Propyl gallate (PG) is one of the most widely used antioxidants in food products, cosmetics and pharmaceutical industries. Increased research has suggested that exposure to PG influences reproductive health in humans and Animals. However, until now, it has not yet been confirmed whether PG would impact oocyte quality. In this study, the hazardous effects of PG on oocyte meiotic maturation were investigated in mice. The findings showed that PG exposure compromises oocyte meiosis by inducing mitochondrial stress which activates Apoptosis to trigger oocyte demise. Moreover, DNA damage was significantly induced in PG-treated oocytes, which might be another cause of oocyte developmental arrest and degeneration. Besides, the level of histone methylation (H3K27me2 and H3K27me3) in oocyte was also significantly increased by PG exposure. Furthermore, PG-induced oxidative stress was validated by the increased level of Reactive Oxygen Species (ROS), which might be the underlying reason for these abnormities. In conclusion, the foregoing findings suggested that PG exposure impaired oocyte meiotic maturation by yielding mitochondrial stress to activate Apoptosis, inducing DNA damage and oxidative stress, and altering histone methylation level.

Keywords

DNA damage; meiotic maturation; mitochondrial stress; mouse oocyte; propyl gallate.

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