2. Academic Validation
  3. Interaction of Fumonisin B1, N-Palmitoyl-Fumonisin B1, 5- O-Palmitoyl-Fumonisin B1, and Fumonisin B4 Mycotoxins with Human Serum Albumin and Their Toxic Impacts on Zebrafish Embryos

Interaction of Fumonisin B1, N-Palmitoyl-Fumonisin B1, 5- O-Palmitoyl-Fumonisin B1, and Fumonisin B4 Mycotoxins with Human Serum Albumin and Their Toxic Impacts on Zebrafish Embryos

  • Biomolecules. 2023 Apr 27;13(5):755. doi: 10.3390/biom13050755.
Zsolt Csenki 1,  Tibor Bartók 2,  Illés Bock 1,  Levente Horváth 2 3,  Beáta Lemli 4 5,  Balázs Zoltán Zsidó 6,  Cserne Angeli 3,  Csaba Hetényi 6,  István Szabó 1,  Béla Urbányi 7,  Melinda Kovács 3 8,  Miklós Poór 4 9
Affiliations

Affiliations

  • 1 Department of Environmental Toxicology, Institute of Aquaculture and Environmental Safety, Hungarian University of Agriculture and Life Sciences, Páter Károly u. 1, H-2100 Gödöllő, Hungary.
  • 2 Fumizol Ltd., Kisfaludy u. 6/B, H-6725 Szeged, Hungary.
  • 3 Institute of Physiology and Nutrition, Agriobiotechnology and Precision Breeding for Food Security National Laboratory, Hungarian University of Agriculture and Life Sciences, Guba Sándor út 40, H-7400 Kaposvár, Hungary.
  • 4 Department of Pharmacology, Faculty of Pharmacy, University of Pécs, Rókus u. 2, H-7624 Pécs, Hungary.
  • 5 Green Chemistry Research Group, János Szentágothai Research Centre, University of Pécs, Ifjúság útja 20, H-7624 Pécs, Hungary.
  • 6 Department of Pharmacology and Pharmacotherapy, Pharmacoinformatics Unit, Medical School, University of Pécs, Szigeti út 12, H-7624 Pécs, Hungary.
  • 7 Department of Aquaculture, Institute of Aquaculture and Environmental Safety, Hungarian University of Agriculture and Life Sciences, Páter Károly u. 1, H-2100 Gödöllő, Hungary.
  • 8 ELKH-MATE Mycotoxins in the Food Chain Research Group, Guba Sándor út 40, H-7400 Kaposvár, Hungary.
  • 9 Food Biotechnology Research Group, János Szentágothai Research Centre, University of Pécs, Ifjúság útja 20, H-7624 Pécs, Hungary.
PMID: 37238625 DOI: 10.3390/biom13050755
Abstract

Fumonisins are frequent food contaminants. The high exposure to fumonisins can cause harmful effects in humans and Animals. Fumonisin B1 (FB1) is the most typical member of this group; however, the occurrence of several other derivatives has been reported. Acylated metabolites of FB1 have also been described as possible food contaminants, and the very limited data available suggest their significantly higher toxicity compared to FB1. Furthermore, the physicochemical and toxicokinetic properties (e.g., albumin binding) of acyl-FB1 derivatives may show large differences compared to the parent mycotoxin. Therefore, we tested the interactions of FB1, N-palmitoyl-FB1 (N-pal-FB1), 5-O-palmitoyl-FB1 (5-O-pal-FB1), and fumonisin B4 (FB4) with human serum albumin as well as the toxic effects of these mycotoxins on zebrafish embryos were examined. Based on our results, the most important observations and conclusions are the following: (1) FB1 and FB4 bind to albumin with low affinity, while palmitoyl-FB1 derivatives form highly stable complexes with the protein. (2) N-pal-FB1 and 5-O-pal-FB1 likely occupy more high-affinity binding sites on albumin. (3) Among the mycotoxins tested, N-pal-FB1 showed the most toxic effects on zebrafish, followed by 5-O-pal-FB1, FB4, and FB1. (4) Our study provides the first in vivo toxicity data regarding N-pal-FB1, 5-O-pal-FB1, and FB4.

Keywords

5-O-palmitoyl-fumonisin B1; N-palmitoyl-fumonisin B1; fumonisin B1; fumonisin B4; human serum albumin; zebrafish embryos.

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