Silence of linc00023 inhibits pyroptosis and promotes cell proliferation via regulating p53

Objective: The objective of our study is to investigate the role and potential mechanism of linc00023 in the development of Pyroptosis in clear cell renal cell carcinoma (ccRCC).

Methods: We assessed the expression of linc00023 in cells using qRT-PCR. Following linc00023 knockdown, we monitored cell proliferation and the Pyroptosis marker using MTS, qRT-PCR, western blot analysis, and ELISA assays. Additionally, we performed RNA sequencing after linc00023 knockdown and validated the involvement of p53 using western blot analysis. Furthermore, we evaluated the potential mechanism by assessing cell proliferation and the expression of the Pyroptosis marker after treatment with a p53 activator in linc00023-inhibited cells.

Results: Linc00023 expression was downregulated in ccRCC cells. Among them, ACHN cells exhibited higher linc00023 expression and were selected for further investigation. Knockdown of linc00023 resulted in increased cell proliferation and decreased Pyroptosis. Furthermore, inhibition of linc00023 led to changes in the expression of numerous mRNAs, including p53. Importantly, the p53 activator ReACp53 reversed the effects of linc00023 knockdown on cell proliferation and Pyroptosis.

Conclusion: In conclusion, our findings suggested that linc00023 regulates Pyroptosis in ccRCC by modulating p53 expression.

cell proliferation; clear cell renal cell carcinoma; linc00023; p53; pyroptosis.