2. Academic Validation
  3. Affinity Purification-Mass Spectroscopy (AP-MS) and Co-Immunoprecipitation (Co-IP) Technique to Study Protein-Protein Interactions

Affinity Purification-Mass Spectroscopy (AP-MS) and Co-Immunoprecipitation (Co-IP) Technique to Study Protein-Protein Interactions

  • Methods Mol Biol. 2023:2690:81-85. doi: 10.1007/978-1-0716-3327-4_7.
Prabu Gnanasekaran 1,  Hanu R Pappu 2
Affiliations

Affiliations

  • 1 Department of Plant Pathology, Washington State University, Pullman, WA, USA.
  • 2 Department of Plant Pathology, Washington State University, Pullman, WA, USA. [email protected].
PMID: 37450138 DOI: 10.1007/978-1-0716-3327-4_7
Abstract

Affinity purification-Mass spectroscopy (AP-MS) is a biochemical technique to identify the novel protein-protein interaction that occurs in the most relevant physiological conditions, whereas co-immunoprecipitation (Co-IP) is used to study the interaction between two known protein partners that are expressed in the native physiological conditions. Both AP-MS and Co-IP techniques are based on the ability of the interacting partners to pull-down with protein of interest. In this chapter, we have explained the AP-MS and Co-IP methods to study protein-protein interactions in the plant cells.

Keywords

AP-MS; Co-IP; GFP-trap resins; Immunoglobulin A (IgA) beads.

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