2. Academic Validation
  3. Involvement of skin TRPV3 in temperature detection regulated by TMEM79 in mice

Involvement of skin TRPV3 in temperature detection regulated by TMEM79 in mice

  • Nat Commun. 2023 Jul 20;14(1):4104. doi: 10.1038/s41467-023-39712-x.
Jing Lei 1 2 3,  Reiko U Yoshimoto 4,  Takeshi Matsui 5 6 7,  Masayuki Amagai 5 7,  Mizuho A Kido 4,  Makoto Tominaga 8 9 10
Affiliations

Affiliations

  • 1 Division of Cell Signaling, National Institute for Physiological Sciences, National Institutes of Natural Sciences, 444-8787, Okazaki, Japan.
  • 2 Department of Physiological Sciences, The Graduate University for Advanced Studies (SOKENDAI), 444-8585, Okazaki, Japan.
  • 3 Thermal Biology Group, Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences, 444-8787, Okazaki, Japan.
  • 4 Division of Histology and Neuroanatomy, Department of Anatomy and Physiology, Faculty of Medicine, Saga University, 849-8501, Saga, Japan.
  • 5 Laboratory for Skin Homeostasis, RIKEN Center for Integrative Medical Sciences, 230-0045, Yokohama, Japan.
  • 6 Laboratory for Evolutionary Cell Biology of the Skin, School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 Katakura, Hachioji, 192-0982, Tokyo, Japan.
  • 7 Department of Dermatology, Keio University School of Medicine, 160-8582, Tokyo, Japan.
  • 8 Division of Cell Signaling, National Institute for Physiological Sciences, National Institutes of Natural Sciences, 444-8787, Okazaki, Japan. [email protected].
  • 9 Department of Physiological Sciences, The Graduate University for Advanced Studies (SOKENDAI), 444-8585, Okazaki, Japan. [email protected].
  • 10 Thermal Biology Group, Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences, 444-8787, Okazaki, Japan. [email protected].
PMID: 37474531 DOI: 10.1038/s41467-023-39712-x
Abstract

TRPV3, a non-selective cation transient receptor potential (TRP) ion channel, is activated by warm temperatures. It is predominantly expressed in skin keratinocytes, and participates in various somatic processes. Previous studies have reported that thermosensation in mice lacking TRPV3 was impaired. Here, we identified a transmembrane protein, TMEM79, that acts as a negative regulator of TRPV3. Heterologous expression of TMEM79 was capable of suppressing TRPV3-mediated currents in HEK293T cells. In addition, TMEM79 modulated TRPV3 translocalization and promoted its degradation in the lysosomes. TRPV3-mediated currents and CA2+ influx were potentiated in primary mouse keratinocytes lacking TMEM79. Furthermore, TMEM79-deficient male mice preferred a higher temperature than did wild-type mice due to elevated TRPV3 function. Our study revealed unique interactions between TRPV3 and TMEM79, both in vitro and in vivo. These findings support roles for TMEM79 and TRPV3 in thermosensation.

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