2. Academic Validation
  3. Endocytic vesicles act as vehicles for glucose uptake in response to growth factor stimulation

Endocytic vesicles act as vehicles for glucose uptake in response to growth factor stimulation

  • bioRxiv. 2023 Jul 24:2023.07.23.550235. doi: 10.1101/2023.07.23.550235.
Ryouhei Tsutsumi 1 2 3,  Beatrix Ueberheide 3 4 5,  Feng-Xia Liang 3 6,  Benjamin G Neel 3,  Ryuichi Sakai 1,  Yoshiro Saito 2
Affiliations

Affiliations

  • 1 Kitasato University School of Medicine; Sagamihara 252-0374, Kanagawa, Japan.
  • 2 Graduate School of Pharmaceutical Sciences, Tohoku University; Sendai 980-8578, Miyagi, Japan.
  • 3 Perlmutter Cancer Center, NYU Grossman School of Medicine, NYU Langone Health; New York, NY 10016, USA.
  • 4 Proteomics Laboratory, NYU Grossman School of Medicine, NYU Langone Health; New York, NY 10016, USA.
  • 5 Department of Biochemistry and Molecular Pharmacology and Department of Neurology, NYU Grossman School of Medicine, NYU Langone Health; New York, NY 10016, USA.
  • 6 Microscopy Laboratory, NYU Grossman School of Medicine, NYU Langone Health; New York, NY 10016, USA.Paste the full affiliation list here.
PMID: 37546742 DOI: 10.1101/2023.07.23.550235
Abstract

Glycolysis is a fundamental cellular process, yet its regulatory mechanisms remain incompletely understood. Here, we show that a subset of glucose transporter 1 (GLUT1/SLC2A1) co-endocytoses with platelet-derived growth factor (PDGF) receptor (PDGFR) upon PDGF-stimulation. Furthermore, multiple glycolytic Enzymes localize to these endocytosed PDGFR/GLUT1-containing vesicles adjacent to mitochondria. Contrary to current models, which emphasize the importance of glucose transporters on the cell surface, we find that PDGF-stimulated glucose uptake depends on receptor/transporter endocytosis. Our results suggest that growth factors generate glucose-loaded endocytic vesicles that deliver glucose to the glycolytic machinery in proximity to mitochondria, and argue for a new layer of regulation for glycolytic control governed by cellular membrane dynamics.

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