2. Academic Validation
  3. GABA regulates the proliferation and apoptosis of head and neck squamous cell carcinoma cells by promoting the expression of CCND2 and BCL2L1

GABA regulates the proliferation and apoptosis of head and neck squamous cell carcinoma cells by promoting the expression of CCND2 and BCL2L1

  • Life Sci. 2023 Oct 20:122191. doi: 10.1016/j.lfs.2023.122191.
Kunliang Luo 1,  Xiangtong Zhao 2,  Yidan Shan 3,  Xuewen Wang 4,  Yaohan Xu 4,  Ming Chen 5,  Qingqing Wang 6,  Yinjing Song 7
Affiliations

Affiliations

  • 1 Department of Dentistry, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310016, China; Department of Oral and Maxillofacial Surgery, University Hospital of Schleswig-Holstein, Campus Kiel, Arnold-Heller-Straße 3, 24105 Kiel, Germany.
  • 2 Institute of Immunology, Zhejiang University School of Medicine, Hangzhou 310058, China.
  • 3 Department of Oral and Maxillofacial Surgery, The Second Affiliate Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
  • 4 Department of Dermatology and Venereology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310016, China.
  • 5 Department of Medical Oncology, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China. Electronic address: [email protected].
  • 6 Institute of Immunology, Zhejiang University School of Medicine, Hangzhou 310058, China. Electronic address: [email protected].
  • 7 Department of Dermatology and Venereology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310016, China. Electronic address: [email protected].
PMID: 37866807 DOI: 10.1016/j.lfs.2023.122191
Abstract

Gamma-aminobutyric acid (GABA) is a multifunctional molecule that is widely present in the nervous system and nonneuronal tissues. It plays pivotal roles in neurotransmission, regulation of secretion, cell differentiation, proliferation, and tumorigenesis. However, the exact mechanisms of GABA in head and neck squamous cell carcinomas (HNSCCs) are unknown. We took advantage of RNA sequencing in this work and uncovered the potential gene expression profiles of the GABA-treated HNSCC cell line HN4-2. We found that the expression of CCND2 and BCL2L1 was significantly upregulated. Furthermore, GABA treatment inhibited the cell Apoptosis induced by cisplatin and regulated the cell cycle after treatment with cisplatin in HN4-2 cells. Moreover, we also found that GABA could upregulate the expression of CCND2 and BCL2L1 after treatment with cisplatin. Our results not only reveal the potential pro-tumorigenic effect of GABA on HNSCCs but also provide a novel therapeutic target for HNSCC treatment.

Keywords

Apoptosis; BCL2L1; CCND2; GABA; HNSCCs; Proliferation.

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