2. Academic Validation
  3. Diallyl trisulfide improves spinal cord ischemia-reperfusion injury damage by activating AMPK to stabilize mitochondrial function

Diallyl trisulfide improves spinal cord ischemia-reperfusion injury damage by activating AMPK to stabilize mitochondrial function

  • J Orthop Surg Res. 2023 Nov 6;18(1):838. doi: 10.1186/s13018-023-04176-8.
Yang Sun # 1 2,  Dengyue Xu # 3,  Weidong Yang 1,  Hongquan Zhang 1,  Yi Su 1,  Bin Gao 1,  Xiaowei Zou 1,  Yiming Zhong 1,  Huanwei Sun 4,  Liangbi Xiang 5
Affiliations

Affiliations

  • 1 Department of Hand and Foot Surgery, Central Hospital of Dalian University of Technology, No. 826, Southwest Road, Shahekou District, Dalian, 116000, Liaoning Province, People's Republic of China.
  • 2 Postgraduate College, China Medical University, No. 77, Puhe Road, New Shenbei District, Shenyang, 110122, Liaoning Province, People's Republic of China.
  • 3 School of Biomedical Engineering, Faculty of Medicine, Dalian University of Technology, No. 2, Linggong Road, Ganjingzi District, Dalian, 116024, Liaoning Province, People's Republic of China.
  • 4 Department of Hand and Foot Surgery, Central Hospital of Dalian University of Technology, No. 826, Southwest Road, Shahekou District, Dalian, 116000, Liaoning Province, People's Republic of China. [email protected].
  • 5 Department of Orthopedics, General Hospital of Northern Theater Command, No. 83, Wenhua Road, Shenhe District, Shenyang, 110000, Liaoning Province, People's Republic of China. [email protected].
  • # Contributed equally.
PMID: 37932742 DOI: 10.1186/s13018-023-04176-8
Abstract

Background: Spinal cord ischemia-reperfusion injury (SCII) is a catastrophic event, which can cause paraplegia in severe cases. In the reperfusion stage, oxidative stress was up-regulated, which aggravated the injury and Apoptosis of neurons. As the main active ingredient of garlic, diallyl trisulfide (DATS) displays strong antioxidant capacity. However, it is unknown whether DATS can protect the neurons of SCII.

Materials and methods: In this study, the descending aorta at the distal end of the left subclavian artery was ligated and perfused again after 14 min. Samples including blood and spinal cord (L2-L5) were taken 24 h later for morphological and biochemical examination.

Results: After SCII, the rats showed motor dysfunction, increase Apoptosis, malondialdehyde content, mitochondrial biogenesis and dynamic balance disorder. After the application of DATS, the adenosine monophosphate activated protein kinase (AMPK) was activated, the mitochondrial damage was improved, the oxidative stress was weakened, and the neuronal damage was recovered to some extent. However, the addition of compound C significantly weakened the protective effect of DATS.

Conclusion: Oxidative stress caused by mitochondrial damage was one of the important mechanisms of neuronal damage in SCII. DATS could activate AMPK, stabilize mitochondrial biogenesis and dynamic balance, and reduce neuronal damage caused by oxidative stress.

Keywords

Adenosine monophosphate activated protein kinase; Compound C; Diallyl trisulfide; Mitochondrial function; Spinal cord ischemia–reperfusion injury.

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