2. Academic Validation
  3. Monaspin B, a Novel Cyclohexyl-furan from Cocultivation of Monascus purpureus and Aspergillus oryzae, Exhibits Potent Antileukemic Activity

Monaspin B, a Novel Cyclohexyl-furan from Cocultivation of Monascus purpureus and Aspergillus oryzae, Exhibits Potent Antileukemic Activity

  • J Agric Food Chem. 2024 Jan 17;72(2):1114-1123. doi: 10.1021/acs.jafc.3c08187.
Zitong Meng 1 2,  Yingao Tan 1 3,  Ya-Li Duan 1 3,  Mu Li 1 3
Affiliations

Affiliations

  • 1 Hubei International Scientific and Technological Cooperation Base of Traditional Fermented Foods, Key Laboratory of Environment Correlative Dietology, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei Province 430070, China.
  • 2 Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, Tongji Medical College, Huazhong University of Science and Technology, Hangkong Road 13, Wuhan 430030, China.
  • 3 College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei Province 430070, China.
PMID: 38166364 DOI: 10.1021/acs.jafc.3c08187
Abstract

Natural Products are a rich resource for the discovery of innovative drugs. Microbial cocultivation enables discovery of novel Natural Products through tandem enzymatic catalysis between different fungi. In this study, Monascus purpureus, as a food fermentation strain capable of producing abundant Natural Products, was chosen as an example of a cocultivation pair strain. Cocultivation screening revealed that M. purpureus and Aspergillus oryzae led to the production of two novel cyclohexyl-furans, Monaspins A and B. Optimization of the cocultivation mode and media enhanced the production of Monaspins A and B to 1.2 and 0.8 mg/L, respectively. Monaspins A and B were structurally elucidated by HR-ESI-MS and NMR. Furthermore, Monaspin B displayed potent antiproliferative activity against the leukemic HL-60 cell line by inducing Apoptosis, with a half-maximal inhibitory concentration (IC50) of 160 nM. Moreover, in a mouse leukemia model, Monaspin B exhibited a promising in vivo antileukemic effect by reducing white blood cell, lymphocyte, and neutrophil counts. Collectively, these results indicate that Monaspin B is a promising candidate agent for leukemia therapy.

Keywords

Aspergillus oryzae; Monascus purpureus; cocultivation; leukemia; natural products.

Figures
Products