2. Academic Validation
  3. Discovery of Kebanmycins with Antibacterial and Cytotoxic Activities from the Mangrove-Derived Streptomyces sp. SCSIO 40068

Discovery of Kebanmycins with Antibacterial and Cytotoxic Activities from the Mangrove-Derived Streptomyces sp. SCSIO 40068

  • J Nat Prod. 2024 Jun 28;87(6):1591-1600. doi: 10.1021/acs.jnatprod.4c00232.
Mengran Zhao 1 2 3,  Wenjun Zhang 1 2 3,  Chunfang Yang 1 2 3,  Liping Zhang 1 2 3,  Huarong Huang 4,  Yiguang Zhu 1 2 3,  Disna Ratnasekera 5,  Changsheng Zhang 1 2 3
Affiliations

Affiliations

  • 1 Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China.
  • 2 Sanya Institute of Ocean Eco-Environmental Engineering, Sanya 572000, China.
  • 3 University of Chinese Academy of Sciences, Beijing 100049, China.
  • 4 School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, China.
  • 5 Department of Agricultural Biology, Faculty of Agriculture, University of Ruhuna, Kamburupitiya 81000, Sri Lanka.
PMID: 38862138 DOI: 10.1021/acs.jnatprod.4c00232
Abstract

Mangrove derived actinomycetes are a rich reservoir of bioactive natural products and play important roles in pharmaceutical chemistry. In a screen of actinomycetes from mangrove rhizosphere sedimental environments, the isolated strain Streptomyces sp. SCSIO 40068 displayed strong Antibacterial activity. Further fractionation of the extract yielded four new compounds kebanmycins A-D (1-4) and two known analogues FD-594 (5) and the aglycon (6). The structures of 1-6 were determined based on extensive spectroscopic data and single-crystal X-ray diffraction analysis. 1-3 featured a fused pyranonaphthaxanthene as an integral part of a 6/6/6/6/6/6 polycyclic motif, and showed bioactivity against a series of Gram-positive bacteria and cytotoxicity to several human tumor cells. In addition, the kebanmycins biosynthetic gene cluster (keb) was identified in Streptomyces sp. SCSIO 40068, and KebMT2 was biochemically characterized as a tailoring sugar-O-methyltransferase, leading to a proposed biosynthetic route to 1-6. This study paves the way to further investigate 1 as a potential lead compound.

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